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Master's Dissertation
DOI
10.11606/D.42.2009.tde-01022010-091619
Document
Author
Full name
Luciana Ahlf Bandini
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2009
Supervisor
Committee
Gennari, Solange Maria (President)
Labruna, Marcelo Bahia
Schumaker, Teresinha Tizu Sato
Title in Portuguese
Infecção experimental de cães (Canis familiaris) com oocistos esporulados de Neospora caninum.
Keywords in Portuguese
Neospora caninum
Cães
Infecção experimental animal
Oócitos
Abstract in Portuguese
O objetivo deste estudo foi infectar experimentalmente cães com oocistos esporulados de N. caninum, via oral e avaliar a ocorrência ou não da infecção. Os oocistos utilizados como inóculo foram obtidos através de bioensaio em cães, usando cérebro de búfalos soropositivos para anticorpos anti-N. caninum. Os oocistos obtidos foram confirmados ser de N. caninum por métodos moleculares e por bioensaio em gerbilos. Oocistos esporulados, com no máximo 90 dias, foram utilizados na infecção de quatro cães, com oito semanas de vida e negativos para anticorpos anti-N. caninum e Toxoplasma gondii. Os cães 1 e 4 receberam um inóculo com 10.000 oocistos esporulados cada um; o cão 2 um inóculo com 5.000 oocistos esporulados e o cão 3 recebeu 1.000 oocistos esporulados de N. caninum. O total de fezes foi coletado e examinado diariamente durante um período de 30 dias. Nenhum oocisto foi encontrado nas fezes desses animais. Durante seis meses os cães foram acompanhados para observar uma possível soroconversão e quando esta ocorria os animais eram eliminados do experimento.
Title in English
Exprimental infection of dogs (Canis familiaris) with sporulated oocysts of Neospora caninum.
Keywords in English
Neospora caninum
Dogs
Experimental animal infection
Oocytes
Abstract in English
The objective of this study was to evaluate the acquisition of infection in dogs experimentally infected with sporulated oocysts. The oocysts used as inoculum were obtained by bioassay in dogs using brain of buffaloes positive for anti-N. caninum antibodies. The oocysts were confirmed to be N. caninum by molecular methods and by bioassay in gerbils. Sporulated oocysts with a maximum of 90 days were used in the infection of four dogs. The dogs were eight weeks old and were negative for anti-N. caninum and Toxoplasma gondii antibodies. Dogs 1 and 4 received an inoculum of 10,000 sporulated oocysts each, dog 2 an inoculum with 5,000 sporulated oocysts and dog 3 received 1,000 sporulated oocysts of N. caninum. The total feces eliminated by the dogs were collected and examined daily for a period of 30 days. No oocysts were found in the feces of these animals. For six months the dogs were monitored to observe a possible seroconversion and when this occurred the animals were eliminated from the experiment.
 
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Publishing Date
2010-03-01
 
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