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Doctoral Thesis
DOI
10.11606/T.42.2014.tde-25112014-110540
Document
Author
Full name
Maraysa de Oliveira Melo
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2014
Supervisor
Committee
Yan, Chao Yun Irene (President)
Almeida, Federico David Brown
Capelo, Luciane Portas
Jaeger, Ruy Gastaldoni
Xavier Neto, José
Title in Portuguese
O papel do compexo PAR durante a embriogênese do placóide do cristalino.
Keywords in Portuguese
Actina
Embrião de galinha
Placoide do cristalino
Polarização celular
Proteínas PAR
Abstract in Portuguese
O cristalino se origina de um epitélio simples e cuboidal que recobre a vesícula óptica. Neste estádio, os filamentos de actina são distribuídos ao longo do eixo apicobasal. As células do ectoderma pré- placodal, em contato com a vesícula óptica, formam um epitélio pseudoestratificado, chamado de placóide do cristalino, com acúmulo de actina no domínio apical. Nós propusemos estudar o papel da proteína PAR3 e sua fosforilação no estabelecimento de actina apical. A superexpressão de PAR3 no placóide forma pontos ectópicos de PAR3 na membrana baso-lateral e induz o recrutamento de actina ectópica para esses pontos. O recrutamento de actina e aPKC ectópicos é independente do estado de fosforilação da treonina 833, resíduo localizado no domínio de ligação do PAR3 ao aPKC. Além disso, no ectoderma peri-placoidal, onde a actina localiza-se baso-lateralmente, PAR3 induz o recrutamento ectópico de actina apical e esse recrutamento é independente da fosforilação da treonina 833. Esses dados nos sugerem que PAR3 é suficiente para recrutar actina no placóide do cristalino.
Title in English
The role of PAR complex during lens placode embryogenesis.
Keywords in English
Actin
Cell polarization
Chicken embryo
Lens placode
PAR proteins
Abstract in English
The lens originates from a simple cuboidal epithelium that overlies the optic vesicle. At this stage, the actin filaments are distributed along its apical-basal sides. The pre-placodal ectoderm, in contact with the optic vesicle, forms a pseudostratified tissue, the lens placode, with accumulation of actin network at the apical domain. Here, we focused on the role of the polarity protein PAR3 and its phosphorylation in the establishment of this apical actin network. Overexpression of PAR3 in the lens placode, induced formation of ectopic actin clusters in the basolateral membrane of the lens placode. The formation of these actin clusters, as well as recruitment of aPKC was independent of Threonine 833 phosphorylation at the PAR3 aPKC-binding site. In addition, PAR3 induced ectopic actin networks in the apical membrane of the periplacodal ectoderm independent of the Threonine 833 phosphorylation. Taken together, these data suggest that PAR3 is sufficient for actin recruitment in the lens placode.
 
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Release Date
2018-11-26
Publishing Date
2014-11-27
 
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