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Master's Dissertation
DOI
10.11606/D.42.2014.tde-24092014-135218
Document
Author
Full name
Andrews Krupinski Emerenciano
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2014
Supervisor
Committee
Silva, Jose Roberto Machado Cunha da (President)
Gomes, Vicente
Pimenta, Daniel Carvalho
Title in Portuguese
Extração, purificação e avaliação da atividade fagocítica do equinocromo em ouriços-do-mar Lytechinus variegatus (Lamarck, 1816).
Keywords in Portuguese
Lytechinus variegatus
Equinocromo
Fagocitose
Imunidade inata
Ouriço-do-mar
Abstract in Portuguese
Em ouriços, os esferulócitos vermelhos são responsáveis pela biossíntese do equinocromo, um pigmento naftaquinônico considerado antioxidante e bactericida, no entanto seu papel na resposta imune permanece pouco elucidado. O presente trabalho avaliou a reposta imune inata de ouriços-do-mar Lytechinus variegatus, através da atividade fagocítica frente a diferentes concentrações de equinocromo (50 e 100 µg/ml). Para tanto, o equinocromo foi extraído e purificado por RP-HPLC. Nossos resultados demonstraram que o equinocromo em ambas as concentrações modula positivamente a fagocitose, aumentando a quantidade de células fagocitando. A concentração de 50 µg/ml foi capaz de ativar os amebócitos fagocíticos (AF), e aumentar a quantidade de AF com quatro ou mais leveduras fagocitadas. Já na concentração de 100 µg/ml, além da ativação dos AF, aumentou também, a quantidade de AF com uma, duas, quatro ou mais leveduras fagocitadas, sugerindo uma atuação dose-dependente. Desta forma, os dados apresentados demonstram que o equinocromo exerce um importante papel na resposta imune.
Title in English
Extraction, purification and evaluation of the phagocytic activity of echinochrome in the sea urchins Lytechinus variegatus (Lamarck, 1816).
Keywords in English
Lytechinus variegatus
Echinochrome
Innate immunity
Phagocytosis
Sea urchin
Abstract in English
The biosynthesis of echinochrome is mediated by red sphere cell. This naphthoquinonic e pigment presents antioxidant and bactericidal characteristics. However, the echinochrome role in immune response remains unclear. In this study, we evaluated the innate immune response of the sea urchin Lytechinus variegatus. To this purpose, the echinochrome was extracted and purified by RP-HPLC. Finally, phagocytic amoebocytes were exposed to different concentrations of echinochrome (50 and 100 mg/ml), when phagocytic activity was analysed. Here, we showed that echinochrome positively modulate phagocytosis, increasing the number of phagocytizing cells. The concentration of 50 mg/ml activated phagocytic amoebocytes (AF), and increased the number of AF containing four or more phagocytosed yeasts. For the other hand, at 100 mg/ml exposure, the activation of AF also increased the number of AF with one, two, four or more yeast phagocytosed, suggesting a dose-dependent activity. Thus, the data presented demonstrated that echinochrome plays an important role in the immune response.
 
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Publishing Date
2014-10-07
 
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