Splicing is a critical process for gene expression in eukaryotes, as genes are transcribed as pre-mRNAs, which are composed of exons (sequences that remain in mature mRNAs) and introns (intermediate sequences). The splicing process involves intron excision and junction of exons, resulting in mature transcripts (mRNA). This process is performed by a complex machinery called spliceosome, which is composed by five snRNAs and more than 100 proteins, some of which may transiently associate with spliceosome components or pre-mRNAs. HnRNPs are proteins that can associate with the spliceosome complex, playing a regulatory role at splicing sites and often involved in alternative splicing mediation. In the human genome, over 70% of miRNAs are located in introns, therefore, it is possible that the splicing process is important for their maturation. Increased miR-17-92 cluster expression levels have been related to the development of many pathologies, such as thyroid cancer, lung cancer and lymphoma. Previous studies have shown that hnRNP A1 is important for miR-18a maturation. We investigated the potential association of hnRNP A1 and hnRNP G proteins with miRNAs from the miR-17-92 cluster. The results showed that hnRNP A1 is associated with other members of the miR-17-92 cluster, and that overexpression of this protein increases the proliferative, migratory and invasive capabilities of papillary thyroid cancer cells, BCPAP.