Peptídeo AG73, derivado da laminina-111, induz migração, invasão e secreção de proteases em linhagem celular derivada de carcinoma epidermóide oral através de sindecana-1 e integrina b1

Siqueira, Adriane Sousa de

doi:10.11606/D.42.2009.tde-09022010-104223

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Master's Dissertation

DOI

https://doi.org/10.11606/D.42.2009.tde-09022010-104223

Document

Master's Dissertation

Author

Siqueira, Adriane Sousa de (Catálogo USP)

Full name

Adriane Sousa de Siqueira

Institute/School/College

Instituto de Ciências Biomédicas

Knowledge Area

Cell and Tissue Biology

Date of Defense

2009-09-24

Published

São Paulo, 2009

Supervisor

Jaeger, Ruy Gastaldoni (Catálogo USP)

Committee

Jaeger, Ruy Gastaldoni (President)
Colquhoun, Alison
Correa, Olga Maria de Toledo

Title in Portuguese

Peptídeo AG73, derivado da laminina-111, induz migração, invasão e secreção de proteases em linhagem celular derivada de carcinoma epidermóide oral através de sindecana-1 e integrina b1

Keywords in Portuguese

Carcinoma epidermóide
Integrina \'beta\'1
Laminina
Metaloprotease de matriz
Peptídeo AG73
Sindecan-1

Abstract in Portuguese

Carcinona epidermóide é um prevalente tumor de cabeça e pescoço relacionado a altas taxas de mortalidade. Neste trabalho, verificamos se AG73 (RKRLQVQLSIRT, cadeia a1), peptídeo derivado da laminina-111, regula migração, invasão e secreção de protease em células de carcinoma epidermóide oral (OSCC). Cadeia a1 da laminina e MMP9 estão expressas neste tumor in vivo e in vitro. AG73 induziu aumento da taxa migratória de células OSCC em ensaios de ferida e migração, e também estimulou invasão em ensaio em câmaras bipartites com Matrigel. Células OSCC crescidas sobre AG73 exibiram aumento dose-dependente de MMP9, detectado por zimografia. Buscamos receptores de AG73 que regulariam atividade nesta linhagem. Células OSCC crescidas sobre AG73 exibiram colocalização de sindecana-1 e integrina b1, e silenciamento desses receptores com RNA de interferência promoveu diminuição de migração e invasão dependente de AG73 nestas células. Esses resultados sugerem que sindecana-1 e integrina b1, ativados por AG73, podem regular migração, invasão e secreção de MMPs em células OSCC.

Title in English

Laminin-111-derived peptide AG73 regulates migration, invasion and protease activity of cell line derived from oral squamous cell carcinoma through syndecan-1 and b1 integrin.

Keywords in English

AG73 peptide
Laminin
Matrix metalprotease
Squamous cell carcinoma
Syndecan-1
\'Beta\' 1 integrin

Abstract in English

Oral squamous cell carcinoma is a prevalent head and neck tumor, related to high mortality rates. Here we studied the role played by AG73 (RKRLQVQLSIRT, a1 chain) on migration, invasion and protease secretion of a cell line (OSCC) from human oral squamous cell carcinoma. Laminin a1 chain and MMP9 are expressed in oral squamous cell carcinoma cells in vivo and in vitro. AG73 increased migratory activity of OSCC cells, as shown by monolayer wound assays and migration assays. This peptide also stimulated cell invasion in chemotaxis chambers coated with Matrigel. OSCC cells cultured on AG73 showed a dose-dependent increase of MMP9 secretion, detected by zymography. We searched for AG73 receptors regulating activities in this cell line. OSCC cells grown on AG73 exhibited colocalization of syndecan-1 and b1 integrin, and siRNA knockdown of these receptors decreased AG73-dependent migration and invasion of OSCC cells. Our results suggest that syndecan-1 and b1 integrin signaling downstream of AG73 regulate migration, invasion and MMP secretion by OSCC cells

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Publishing Date

2010-03-15

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