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Master's Dissertation
DOI
10.11606/D.42.2016.tde-18042016-140115
Document
Author
Full name
Denise da Silva Reis
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2015
Supervisor
Committee
Sumida, Telma Miyuki Oshiro (President)
Kaneno, Ramon
Peron, Jean Pierre Schatzmann
Title in Portuguese
Avaliação da expressão da enzima indoleamina 2,3-dioxigenase (IDO) em monócitos e células dendríticas derivadas de monócitos de indivíduos infectados pelo HIV.
Keywords in Portuguese
Células dendríticas
HIV
IDO
Monócitos
Abstract in Portuguese
A análise da expressão da enzima indoleamina 2,3-dioxigenase (IDO), envolvida na regulação da resposta imune, em vacinas de células dendríticas (DCs) como imunoterapia para tratamento de indivíduos HIV+, pode fornecer informações sobre o perfil dessas células e auxiliar o aperfeiçoamento das técnicas atualmente utilizadas. A avaliação da expressão de IDO, por citometria de fluxo, foi realizada em monócitos e DCs de indivíduos sadios e HIV+. A expressão do RNAm de IDO foi analisada por PCR e a capacidade das DCs em estimular linfoproliferação e apresentar antígenos de HIV a linfócitos autólogos foi avaliada por ensaio de cocultivo. DCs ativadas de indivíduos HIV+ demonstraram expressão mais elevada de IDO tanto em relação às DCs imaturas quanto em relação às DCs dos indivíduos sadios. DCs foram capazes de induzir resposta proliferativa e polifuncional de linfócitos autólogos. Nossos resultados sugerem uma expressão diferencial de IDO entre indivíduos sadios e HIV+, indicando um importante papel da enzima no controle da resposta imune e na patogênese da AIDS.
Title in English
Evaluation of indoleamine 2,3-dioxygenase (IDO) expression in monocytes and monocyte derived dendritic cells of HIV patients.
Keywords in English
Dendritic cells
HIV
IDO
Monocytes
Abstract in English
The evaluation of indoleamine 2,3-dioxygenase (IDO) levels, a regulatory enzyme, in the context of DCs vaccines as a therapeutic alternative for the HIV+ patients, can bring information about DCs profiles that can improve current techniques of vaccine production. IDO expression was evaluated by flow cytometry in monocytes and DCs from healthy subjects and HIV+ patients. Expression of IDO mRNA was performed by real-time PCR and the ability in stimulate lymphoproliferation and presenting HIV antigens to autologous lymphocytes was evaluated in coculture assays. Comparison between immature and activated DCs showed an increased IDO expression in activated DCs in patients group. DCs derived from HIV+ patients showed an increased IDO expression when compared to healthy donors. DCs were able to induce lymphofoproliferation and polyfunctional response in autologous lymphocytes. Our results suggest a differential expression of IDO between health subjects and HIV+ patients, indicating an important role of IDO in the control of the immune response and in the HIV pathogenesis.
 
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Publishing Date
2016-04-18
 
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