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Doctoral Thesis
DOI
10.11606/T.42.2014.tde-12122014-124408
Document
Author
Full name
Anderson Daniel Ramos
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2014
Supervisor
Committee
Nunes, Anderson de Sá (President)
Farias, Alessandro dos Santos
Martins, Joilson de Oliveira
Martins, Vania Luiza Deperon Bonato
Ribeiro, José Marcos Chaves
Title in Portuguese
Imunomodulação da encefalomielite autoimune experimental pelo extrato da glândula salivar de Aedes aegypti.
Keywords in Portuguese
Aedes aegypti
Doenças autoimunes
Encefalite autoimune experimental
Extrato da glândula salivar
Imunomodulação
Abstract in Portuguese
A saliva de insetos hematófagos possui moléculas capazes de modular o sistema imune do hospedeiro. Com base na literatura a respeito das atividades presentes na saliva de Aedes aegypti, investigamos se o EGS dessa espécie era capaz de modular a EAE. Imunizamos animais C57BL/6 com MOG35-55, e realizamos um tratamento com EGS. O tratamento com EGS diminuiu a incidência da doença e provocou um atraso no aparecimento dos sinais clínicos, além de estes serem mais brandos. Observamos que a modulação se deu na fase de indução da resposta imune, não na efetora. De fato, o EGS consegue suprimir a doença por 4 vias: 1) diminuindo a expressão de MHCII, CD80 e CD86 em células dendríticas, e diminuindo a produção de citocinas responsáveis pela indução das respostas Th1/Th17; 2) induzindo células produtoras de IL-10 in vivo; 3) induzindo apoptose em linfócitos T naive; 4) induzindo células com perfil Th2 produtoras de IL-4 e IL-5. Concluímos que o EGS é capaz de atuar na supressão dos sintomas durante o curso da EAE e na inibição do início da resposta imune.
Title in English
Immunomodulation of experimental autoimmune encephalomyelitis by salivary gland extract of Aedes aegypti.
Keywords in English
Aedes aegypti
Autoimmune diseases
Experimental autoimmune encephalitis
Immunomodulation
Salivary gland extract
Abstract in English
The saliva of hematophagous insects has molecules that can modulate the host immune system. Based on the literature about activities found in Aedes aegypti saliva, we investigate if SGE of this species could modulate EAE. We have immunized C57BL/6 mice with MOG35-55, and carried out a treatment with SGE. The treatment with SGE reduced the incidence of disease and caused a delay onset of clinical signs making them softer. We have observed that modulation occured in the induction phase of immune response, not in effector phase. In fact, SGE can suppress the disease by four ways: 1) decreasing the expression of MHCII, CD80 and CD86 in dendritic cells and decreasing the production of cytokines responsible for Th1/Th17 response induction; 2) inducing cells producing IL-10 in vivo; 3) inducing apopotosis in naive T lymphocytes; 4) inducing cells Th2 producing IL-4 e IL-5. We came to the conclusion that SGE can act in supressing symptoms during the course of EAE and inhibiting the beggining of autoimmune response.
 
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Release Date
2018-12-15
Publishing Date
2014-12-16
 
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