• JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
 
  Bookmark and Share
 
 
Master's Dissertation
DOI
10.11606/D.42.2014.tde-11072014-094856
Document
Author
Full name
Cristiane Naffah de Souza
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2014
Supervisor
Committee
Bortoluci, Karina Ramalho (President)
Festuccia, William Tadeu Lara
Strauss, Bryan Eric
Title in Portuguese
Regulação da expressão gênica de FASL pela PGE2 em linfócitos T CD4+: papel do repressor transcricional ICER.
Keywords in Portuguese
AICD
FASL
ICER
PGE2
Abstract in Portuguese
Os linfócitos T CD4+ orquestram a resposta imune adaptativa, auxiliando os macrófagos, os linfócitos T CD8+ e os linfócitos B na resposta mais eficiente frente a um antígeno. As etapas que caracterizam uma resposta imune adaptativa são: apresentação do antígeno, ativação e diferenciação dos linfócitos T, expansão clonal e morte celular para retorno à homeostasia via AICD. Este tipo de morte ocorre via FAS/FASL. Tendo em vista que o mecanismo pelo qual a PGE2 inibe a expressão de fasl ainda não é conhecido, o presente trabalho tem como objetivo compreender o mecanismo molecular de atuação da PGE2 sobre os linfócitos T CD4+ na inibição gênica do FASL, tendo como hipótese o envolvimento do repressor transcricional ICER. Foi verificado que PGE2 10-8 M é capaz de proteger as DO11.10 da AICD e que, nesta concentração, ela induz a expressão de icer. Este repressor apresenta uma expressão transiente e observa-se seu aumento concomitantemente à inibição da expressão de fasl. Sendo assim, sugere-se a participação de ICER na via de inibição do fasl pela PGE2.
Title in English
Regulation of FASL gene expression by PGE2 in CD4+ T lymphocytes: role of transcriptional repressor ICER.
Keywords in English
AICD
FASL
ICER
PGE2
Abstract in English
CD4+ T lymphocytes orchestrate the adaptive immune response, helping the macrophages, CD8+ T lymphocytes and B lymphocytes to reach an efficient antigen-specific immune response. The adaptive immune response is characterized by different phases: antigen, T lymphocyte activation and differentiation, clonal expansion and, finally, clonal cell death to return to homeostasis via AICD. This cell death occurs via FAS/FASL. Since the mechanism by which PGE2 inhibits fasl expression is not known, our aim is to understand the molecular mechanism responsible for PGE2 inhibition of fasl in CD4+ lymphocytes. Our hypothesis is that the transcriptional repressor ICER, which binds to CRE sites on gene promoters, is involved in this process. We verified that PGE2 10-8 M protects DO11.10 cells from AICD, and in that concentration, PGE2 increases icer expression. This repressor has a transient expression and we observed that its expression is increased in the same time that fasl expression is inhibited. Thus, we suggest the involvement of ICER in fasl inhibition pathway by PGE2.
 
WARNING - Viewing this document is conditioned on your acceptance of the following terms of use:
This document is only for private use for research and teaching activities. Reproduction for commercial use is forbidden. This rights cover the whole data about this document as well as its contents. Any uses or copies of this document in whole or in part must include the author's name.
Publishing Date
2014-07-11
 
WARNING: Learn what derived works are clicking here.
All rights of the thesis/dissertation are from the authors
Centro de Informática de São Carlos
Digital Library of Theses and Dissertations of USP. Copyright © 2001-2022. All rights reserved.