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Master's Dissertation
DOI
https://doi.org/10.11606/D.42.2009.tde-03022010-093331
Document
Author
Full name
Tatiane Aparecida Canhamero Gasparelo
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2009
Supervisor
Committee
Franco, Marcelo de (President)
Garlet, Gustavo Pompermaier
Isaac, Lourdes
Title in Portuguese
Estudo dos mecanismos genéticos e celulares durante a fase inflamatória do processo de regeneração tecidual em animais selecionados geneticamente para a máxima resposta inflamatória aguda homozigotos para os alelos R ou S do gene Slc11a1.
Keywords in Portuguese
Blastema
Gene Slc11a1
Inflamação (Genética)
Regeneração (Fenômenos biológicos)
Regeneração epimórfica
Regeneração tecidual
Reparo Tecidual
Resposta Inflamatória
Tecido animal (Genética)
Abstract in Portuguese
Sublinhagens de camundongos AIRmax e AIRmin homozigotas para os alelos R ou S do gene Slc11a1 apresentam distinta capacidade regenerativa à perfuração de suas orelhas. Animais AIRmaxSS exibiram regeneração tecidual precoce em comparação aos animais AIRmaxRR, sugerindo que o alelo S favorece a regeneração nestes animais. Camundongos das sublinhagens AIRmin não apresentaram regeneração após perfuração de suas orelhas. Em resposta ao estímulo, animais AIRmaxSS exibiram inflamação local mais intensa e tardia do que animais AIRmaxRR, demonstrando elevados níveis de MPO e edema, e influxo celular predominantemente de neutrófilos. Ensaios de expressão gênica global demonstraram genes diferencialmente expressos entre as sublinhagens, evidenciando genes sobre-representados no tema biológico proliferação celular em ambas sublinhagens, enquanto somente nos animais AIRmaxSS ocorreu sobre-representação para resposta inflamatória nos genes ativados e para contração muscular nos genes reprimidos. Os resultados de microarray foram validados por qPCR.
Title in English
Study of genetic and cellular mechanisms during the inflammatory phase of tissue regeneration process in animals genetically selected for maximum acute inflammatory response homozygous for Slc11a1 R and S alleles.
Keywords in English
Slc11a1
Animal tissue (Genetics)
Blastema
Epimorphic regeneration
Inflammation (Genetics)
Inflammatory response
Regeneration (Biological phenomena)
Tissue regeneration
Tissue repair
Abstract in English
Homozygous AIRmax and AIRmin sublines for Slc11a1 R and S alleles present distinct regenerative capacity to the ear hole. AIRmaxSS mice exhibited early tissue regeneration compared to AIRmaxRR animals, suggesting that the Slc11a1 S allele promotes regeneration in these animals. AIRmin sublines didnt show regeneration after ear punch. In response to the stimulus, AIRmaxSS animals exhibited more intense and later local inflammation than AIRmaxRR animals, presenting elevated levels of MPO, edema and cellular influx predominantly of neutrophils. Global gene expression analysis showed differentially-expressed genes between the sublines, in which over-represented biological theme is cell proliferation in both sublines. AIRmaxSS animals displayed over-representation of inflammatory response in up-regulated genes and of muscle contraction in down-regulated genes. Microarray results were validated by using quantitative PCR.
 
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Publishing Date
2010-03-04
 
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