• JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
 
  Bookmark and Share
 
 
Master's Dissertation
DOI
10.11606/D.42.2013.tde-26062014-112650
Document
Author
Full name
Cristiane Yuri Honna
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2013
Supervisor
Committee
Gomez, José Gregorio Cabrera (President)
Contiero, Jonas
Freire, Denise Maria Guimarães
Title in Portuguese
Obtenção e análise de mutantes de Pseudomonas aeruginosa afetados na biossíntese de ramnolipídeos.
Keywords in Portuguese
Pseudomonas
Ácidos graxos (biossíntese)
Biodegradação
Biossurfactantes
Genética bacteriana
Mutação
Abstract in Portuguese
Ramnolipídeos (RLs) e polihidroxialcanoatos (PHAs) produzidos por Pseudomonas aeruginosa são compostos biodegradáveis e que podem ser obtidos a partir de matérias-primas renováveis. Com o objetivo de elucidar aspectos metabólicos e regulatórios da produção de RLs e PHAs, mutantes de P. aeruginosa LFM634 afetados na síntese desses compostos foram obtidos utilizando um transposon (ISlacZ/hah). Dentre 61 mutantes caracterizados, foram detectados vários super-produtores de RLs e/ou PHAs, bem como alguns nos quais nenhum RL foi detectado. Dois mutantes (p09-13 e p12-39) apresentaram capacidade reduzida de inserir ramnose em RLs e de direcionar intermediários da biossíntese de ácidos graxos para a síntese de RLs ou PHAs. O mutante p09-13 está afetado no gene crc, sugerindo um importante papel deste regulador global no processo de síntese de RLs e PHAs.
Title in English
Obtaining and analysis of mutants of Pseudomonas aeruginosa affected in rhamnolipids biosynthesis.
Keywords in English
Pseudomonas
Bacterial genetics
Biodegradation
Biosurfactants
Fatty acids (biosynthesis)
Mutation
Abstract in English
Rhamnolipids (RLs) and polyhydroxyalkanoates (PHAs) produced by Pseudomonas aeruginosa are biodegradable compounds and can be obtained from renewable raw materials. Aiming to elucidate metabolic and regulatory issues involved in the RLs and PHAs production, P. aeruginosa LFM634 mutants affected in their biosynthesis were obtained using a transposon (ISlacZ/hah). Out of 61mutants characterized, it was detected several overproducing RLs and/or PHA, as well as some in which no RLs was detected. Two mutants (p09-13 and p12-39) presented reduced capability of inserting rhamnose in RLs and of channeling intermediates from fatty acid biosynthesis to the synthesis of RLs and PHAs. The mutant p09-13 is affected on crc gene, suggesting an important role of this global regulator on the process of RLs and PHA synthesis.
 
WARNING - Viewing this document is conditioned on your acceptance of the following terms of use:
This document is only for private use for research and teaching activities. Reproduction for commercial use is forbidden. This rights cover the whole data about this document as well as its contents. Any uses or copies of this document in whole or in part must include the author's name.
Publishing Date
2014-06-28
 
WARNING: Learn what derived works are clicking here.
All rights of the thesis/dissertation are from the authors
Centro de Informática de São Carlos
Digital Library of Theses and Dissertations of USP. Copyright © 2001-2021. All rights reserved.