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Master's Dissertation
DOI
10.11606/D.42.2004.tde-17022005-094136
Document
Author
Full name
Luciano Matsumiya Thomazelli
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2004
Supervisor
Committee
Durigon, Edison Luiz (President)
Lima, Lourdes Rehder de Andrade Vaz de
Pannuti, Claudio Sergio
Title in Portuguese
"Vigilância epidemiológica de vírus respiratórios humanos em amostras clínicas pela técnica de genescan-RT-PCR"
Keywords in Portuguese
diagnóstico rápido
geneScan
metapneumovírus humano
RT-PCR
vírus respiratórios
vírus sincicial respiratório (VSR)
Abstract in Portuguese
As doenças respiratórias agudas (DRAs) são as causas mais comuns de morbidez e mortalidade infantil mundial, podendo ser causadas por uma grande variedade de microorganismos. A fim de se detectar os vírus respiratórios mais comumente associados às infecções agudas do trato respiratório e traçar seu perfil epidemiológico, utilizamos um protocolo de GS RT-PCR (GeneScan Transcrição Reversa-Reação em Cadeia da Polimerase) para a rápida detecção simultânea do, vírus influenza A e B, parainfluenzavirus tipo 1, 2 e 3, picornavirus, metapneumovirus e o adenovírus. As amostras clínicas foram colhidas de crianças menores de cinco anos de idade, apresentando sintomas respiratórios, no Hospital Universitário (HU) da Universidade de São Paulo (USP), durante o ano de 2003. O GS RT-PCR se mostrou uma metodologia sensível e específica, capaz de detectar uma diversidade maior de agentes infecciosos do trato respiratório em relação à Imunofluorescência Indireta (IFI), reduzindo neste estudo a porcentagem de amostras negativas de 69,9% (235 amostras) para 22% (74 amostras).
Title in English
GeneScan Reverse Transcription-PCR assay for surveillance of respiratory viruses in clinical samples of pediatric patients in Brazil
Keywords in English
geneScan
human metapneumovirus
human respiratory syncytial virus (HRSV)
rapid diagnosis
respiratory viruses
RT-PCR
Abstract in English
A reverse transcription polymerase chain reaction (RT-PCR) assay based on automated fluorescent capillary electrophoresis and GeneScan software analysis was used to detect nine common respiratory viruses in clinical specimens from young children. Assays for human respiratory syncytial virus (HRSV), human parainfluenza viruses 1, 2, and 3, influenza A and B viruses, human metapneumovirus, adenovirus and picornavirus were incorporated into a screening PCR standard assay format. The optimized assay panel was used to test 336 respiratory specimens obtained from children hospitalized with acute respiratory illness (ARI) that had been previously tested by viral culture and indirect immunofluorescence staining (IIF). GS RT-PCR showed be a sensitive and specific methodology, able to detect a larger diversity of respiratory viruses regarding IFI, reducing in this study the percentage of negative samples of 69,9% (235 samples) to 22% (74 samples).
 
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Thomazelli.pdf (1.48 Mbytes)
Publishing Date
2005-04-05
 
WARNING: The material described below relates to works resulting from this thesis or dissertation. The contents of these works are the author's responsibility.
  • MURAHOVSCHI, Jayme. Viral respiratory infections : behind simplicity lies intelligence [doi:10.2223/JPED.1758]. Jornal de Pediatria [online], 2012, vol. 84, n. 1, p. 422-8.
  • THOMAZELLI, Luciano M., et al. Surveillance of eight respiratory viruses in clinical samples of pediatric patients in Southeast Brazil [doi:10.2223/JPED.1694]. Jornal de Pediatria [online], 2007, vol. 5, n. 83, p. 422-428.
  • VIEIRA, Sandra. Viral respiratory infections: behind simplicity lies intelligence : Authors' reply. Jornal de Pediatria, 2008, vol. 84, n. 1, p. 95-96.
All rights of the thesis/dissertation are from the authors
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