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Master's Dissertation
DOI
10.11606/D.42.2014.tde-12082014-181231
Document
Author
Full name
Kelli Cardoso Lopes Rodrigues
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2013
Supervisor
Committee
Gomez, José Gregorio Cabrera (President)
Silva, Sonia Regina da
Vasconcellos, Suzan Pantaroto de
Title in Portuguese
Produção de elastômeros biodegradáveis por bactérias isoladas de lodo de esgoto.
Keywords in Portuguese
Pseudomonas sp.
Carboidratos
Metabolismo
Polihidroxialcanoatos
Xilose
Abstract in Portuguese
Este trabalho teve por objetivo avaliar o potencial de 39 isolados bacterianos para produção de polihidroxialcanoatos contendo monômeros de cadeia média (PHA|MCL). Os isolados foram incapazes de utilizar eficientemente amido, lactose ou sacarose. O desempenho na produção de PHA a partir de glicose ou frutose pelos isolados foi inferior à linhagem de referência (Pseudomonas sp. LFM046). Muitos dos isolados apresentaram bom desempenho na conversão de glicerol em PHAMCL. Pela primeira vez, foram detectadas bactérias capazes de produzir PHAMCL a partir de xilose. Com base no sequenciamento do rDNA 16S, estes isolados foram identificados como pertencentes ao gênero Pseudomonas. Cultivos em biorreator, resultaram em uma biomassa de aproximadamente 6 g/L, contendo cerca de 11 a 14% de PHA. A superexpressão dos genes responsáveis pelo catabolismo de xilose (xylAB) em Escherichia coli não levou a melhora no consumo de xilose ou produção de PHAMCL.
Title in English
Production of biodegradable elastomers by bacteria isolated from sewage sludge.
Keywords in English
Pseudomonas sp.
Carbohydrate
Metabolism
Polyhydroxyalkanoates
Xylose
Abstract in English
This work aimed to evaluate the potential of 39 bacterial isolates to produce polyhydroxyalkanoates containing medium-chain monomers (PHAMCL). The isolates were unable to efficiently utilize starch, lactose or sucrose. The performance of the PHA production from glucose or fructose by the isolates was lower than the reference strain (Pseudomonas sp. LFM046). Many isolates showed good performance in the conversion of glycerol into PHAMCL. For the first time, bacterial isolates capable of producing PHAMCL from xylose were detected. Based on the sequencing of 16S rDNA, these isolates were identified as belonging to the genus Pseudomonas. Bioreactor cultures resulted in a biomass of approximately 6 g/L, containing about 11 to 14% PHA. Overexpression of the genes responsible for the catabolism of xylose (xylAB) in Escherichia coli did not led to improved xylose consumption or production of PHAMCL.
 
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Release Date
2018-08-11
Publishing Date
2014-08-14
 
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