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Master's Dissertation
DOI
10.11606/D.42.2014.tde-12082014-175455
Document
Author
Full name
Bruna Prati
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2014
Supervisor
Committee
Pierulivo, Enrique Mario Boccardo (President)
Pinto, Nadja Cristhina de Souza
Termini, Lara
Title in Portuguese
Expressão de genes de vias de reparo de dano ao DNA em células infectadas por papilomavírus humano (HPV).
Keywords in Portuguese
Câncer do colo do útero
Expressão gênica
HPV
Reparo do DNA
Abstract in Portuguese
Os papilomavírus humanos são vírus de DNA que infectam epitélios em regiões anatômicas específicas. Alguns tipos de HPV, coletivamente denominados de alto risco oncogênico, têm associação etiológica com o câncer do colo do útero. Estes vírus expressam dois oncogenes, E6 e E7, que alteram o ciclo celular e o programa de diferenciação das células. Isto promove o acúmulo de defeitos mitóticos e instabilidade genômica, contribuindo à transformação maligna. Alterações nos sistemas de reparo de dano ao DNA associadas à presença de HPV têm sido descritas em diferentes modelos experimentais, no entanto, não tem sido analisadas de maneira sistemática. No presente estudo, avaliamos a expressão de 135 genes envolvidos nas vias de reparo de dano ao DNA em queratinócitos primários humanos e em linhagens derivadas de carcinomas do colo do útero positivas e negativas para HPV. Nossos resultados indicam a presença de alterações importantes na expressão de genes envolvidos nas vias de reparo de dano ao DNA em linhagens derivadas de tumores do colo do útero.
Title in English
Expression of DNA damage repair pathways associated genes in cells infected with human papillomavirus (HPV).
Keywords in English
Cervical cancer
DNA repair
Gene expression
HPV
Abstract in English
Human papillomaviruses are DNA viruses that infect epithelia in specific anatomical regions. Some HPV types, collectively known as high-risk types are etiologically associated with cervical cancer. These viruses express two oncogenes E6 and E7 that alter the cell cycle and cell differentiation. Besides, they promote genomic instability and the accumulation of mitotic defects contributing to malignant transformation. Alterations in the DNA damage repair systems associated with HPV presence have been described in various experimental model systems. However, they have not been systematically analyzed. In this study, we evaluated the expression of 135 genes involved in the DNA damage repair pathways in primary human keratinocytes and cervical cancer derived cell lines. Our results show the presence of important alterations in the expression of DNA damage repair genes in cervical cancer derived cell lines.
 
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Publishing Date
2014-08-14
 
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