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Doctoral Thesis
DOI
10.11606/T.42.2011.tde-11082011-101311
Document
Author
Full name
Jansen de Araujo
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2011
Supervisor
Committee
Durigon, Edison Luiz (President)
Jerez, José Antonio
Labruna, Marcelo Bahia
Levi, José Eduardo
Nogueira, Mauricio Lacerda
Title in Portuguese
Detecção molecular de hantavírus pela técnica de real-time PCR em amostras de roedores silvestres coletadas na região do Vale do Ribeira no Estado de São Paulo.
Keywords in Portuguese
Hanta vírus
Reação em cadeia por polimerase
Roedores
Vale do Ribeira (SP)
Virologia molecular
Abstract in Portuguese
O conhecimento sobre hantavirus patogênicos no Brasil até o momento indica que roedores silvestres são os reservatórios naturais. Estes roedores podem eliminar grandes quantidades do vírus através das fezes, urina e saliva (Khaiboullina et al., 2005). A infecção humana pode ocorrer por contato com roedores ou inalação dos aerossóis que contêm o vírus. A síndrome cardiopulmonar por hantavirus (HCPS) tem sido relatada em humanos em muitas regiões do Brasil. Tendo em vista a quantidade de casos de doenças emergentes e re-emergentes nas últimas décadas elegemos algumas regiões para um estudo detalhado sobre a distribuição desses reservatórios virais e consequentemente a caracterização da variante circulante. Com objetivo de adquirir um diagnóstico rápido e sensível, desenvolvemos primers específicos que pela técnica de Real- time RT- PCR é capaz de detectar o genoma viral em apenas uma hora em amostras de pulmões, rins e urina de roedores. Ao todo, nosso trabalho analisou 153 amostras sendo: 126 de roedores silvestres e domésticos, (de cinco diferentes regiões do Estado de São Paulo onde: 10 amostras colhidas no município de Jacupiranga, 61 oriundas de Teodoro Sampaio, 7 de Salesópolis, 48 provenientes de Biritiba Mirim, 16 de Nazaré Paulista) e mais 27 soros de pacientes de diferentes localidades de Minas Gerais. Neste estudo foi possível detectar dez amostras positivas em Biritiba Mirim, quatro em Nazaré Paulista e em vinte e dois soros de pacientes com hantavirose. O monitoramento contínuo através de testes moleculares em animais silvestres é imprescindível para um melhor entendimento da circulação dos hantavirus nessas regiões. Acreditamos que este teste possa auxiliar na vigilância e também no diagnóstico dos hantavirus no Brasil.
Title in English
Molecular detection of hantavirus by Real- time PCR in wild rodents collected on Vale do Ribeira region, São Paulo State.
Keywords in English
Hanta virus
Molecular Virology
Polymerase chain reaction
Rodents
Vale do Ribeira (SP)
Abstract in English
Current knowledge of the pathogenic hantavirus indicates that wild rodents are its primary natural reservoir. These rodents can eliminate large amounts of the virus through feces, urine and saliva. Human infection can occur through contact with rodents or inhalation of aerosols containing of the virus. Hantavirus cardiopulmonary syndrome (HCPS) has been reported in humans in many regions of Brazil. In view the number of cases of emerging diseases and re- emerging in recent decades, some regions to were select a detailed study on the distribution of these viral reservoirs, and consequently viral characterization. We developed specific primers to detect the presence of viral genomes using Real- time RT- PCR (Reverse- Transcriptase Polymerase Chain Raction). Altogether, our study analyzed 153 samples: 126 of wild rodents and domestic, of five different regions of São Paulo. 10 samples collected in Jacupiranga municipality, 61 in Teodoro Sampaio, 7 Salesópolis, 48 samples in Biritiba Mirim, 16 of Nazaré Paulista and 27 samples of the serum from patients from different sites of Minas Gerais State. Their samples were tested for hantavirus using the described SYBR Green-based real-time RT-PCR protocol and the specific primers. The presence of hantavirus RNA was detected in 10 rodents from Biritiba Mirim, 4 in Nazaré Paulista and 22 in serum of patients with hantaviruse. The surveillance by molecular tests in wild animals is essential to understood of hantavirus circulation in these regions. This SYBR Green real-time RTPCR method for detection of hantavirus may be useful for surveying hantaviruses in Brazil.
 
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Publishing Date
2011-08-24
 
WARNING: The material described below relates to works resulting from this thesis or dissertation. The contents of these works are the author's responsibility.
  • ARAUJO, J., et al. Detection of hantaviruses in Brazilian rodents by SYBR-Green-based real-time RT-PCR [doi:10.1007/s00705-011-0968-1]. Archives of Virology [online], 2011, vol. 156, n. 7, p. 1269-1274.
  • DE ARAUJO, Jansen, et al. Detection of hantavirus in bats from remaining rain forest in São Paulo, Brazil [doi:10.1186/1756-0500-5-690]. BMC Research Notes [online], 2012, vol. 5, n. 1, p. 690.
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