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Doctoral Thesis
DOI
10.11606/T.42.2009.tde-03122009-124057
Document
Author
Full name
Hildenêr Nogueira de Lima e Silva
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2009
Supervisor
Committee
Durigon, Edison Luiz (President)
Arruda Neto, Eurico de
Casseb, Jorge Simão do Rosário
Mehnert, Dolores Ursula
Passos, Saulo Duarte
Title in Portuguese
Variabilidade genética da proteína SH (Small hydrophobic protein) do vírus sincicial respiratório humano isolado de crianças na cidade de São Paulo.
Keywords in Portuguese
Crianças
Filogenia
Microbiologia
Proteína SH
Proteínas G
Respiração
Variação genética
Virologia médica
Vírus sincicial respiratório
Abstract in Portuguese
O vírus sincicial respiratório humano (VSRH) é o agente viral mais freqüentemente relacionado a doenças do trato respiratório inferior em crianças abaixo de um ano de idade. Analíse da varibilidade antigênica e gênica mostraram que o VSRH pode ser divido em dois grupos: A e B. O vírus é um membro do gênero Pneumovirus pertencente a família Paramyxoviridea, e possui três principais proteínas que são: glicoproteina F (fusão), glicoproteina G (adesão), glicoproteina SH (pequena proteína hidrofóbica). A proteína F é responsável pela fusão da célula ao vírus, enquanto a proteína G tem papel fundamental na replicação do vírus, porém a função da proteína SH, ainda não está bem definida, estudos recentes mostram-na como responsável por inibir a sinalização do fator de necrose tumoral alfa (TNF-a). Neste estudo foram colhidas amostras de 965 crianças, entre os anos de 2004 e 2005, dentre as quais 424 foram positivas. 117 amostras foram seqüenciadas a proteína SH e G e comparadas com amostras que circularam mundialmente. A analíse filogenética mostrou uma baixa variabilidade entre os genótipos estudados tanto do grupo A quanto do B.
Title in English
Genetic variability of protein SH of human respiratory syncytial virus (HRSV) of samples collected the children in São Paulo City.
Keywords in English
Breath
Children
Genetic variation
Medical Virology
Microbiology
Phylogeny
Protein G
Protein SH
Respiratory syncytial virus
Abstract in English
The human respiratory syncytial virus (HRSV) is the major cause of lawer respiratory tract infections in infantis, young children and elderly. Analysis of the antigenic and genetic variability has shown that there are two groups of the virus HRSV, A and B. The virus (HRSV) is a member of the genus pneumovirus in the paramyxoviridae family. The virus encodes three membrane-bound glicoproteins, namely the fusion (F) attachment (G) and small hydrophobic (SH) proteins. The F mediates fusion of the virus and cell membranes and the G proteins is involved in virus attachment. The biological properties of the F and G glicoproteins and role that they play during virus replication relatively well understood, however the functional significance of the SH protein during replication remains unclear, although recent study shown that it can inhibit TNF-alpha. In this study, HRSV strains were isolated from nasopharyngeal aspirates collected from 965 children between 2004 and 2005, yielding 424 positive samples. We sequenced the small hydrophobic protein (SH) gene and protein (G) of 117 samples and compared them with other viruses identified worldwide. The phylogenetic analysis showed a low genetic variably among the isolates but allowed us to classify the viruses into different genotypes for the A and B HRSV strains.
 
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Publishing Date
2010-03-02
 
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