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Doctoral Thesis
DOI
10.11606/T.42.2015.tde-27082015-173311
Document
Author
Full name
Marcelo Gomes Pereira
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2015
Supervisor
Committee
Miyabara, Elen Haruka (President)
Bacurau, Reury Frank Pereira
Chaves, Maria Luiza Morais Barreto de
Lancha Junior, Antonio Herbert
Marques, Maria Julia
Title in Portuguese
Efeito da suplementação com leucina no processo de regeneração muscular esquelética.
Keywords in Portuguese
Lesão muscular esquelética
Regeneração
Suplementação com leucina
Abstract in Portuguese
A suplementação com leucina pode ser uma forma de terapia importante para a regeneração do tecido muscular lesado, pois modula vias intracelulares reguladoras da massa muscular. Neste estudo, ratos Wistar tiveram os músculos soleus e TA da pata esquerda submetidos à criolesão. Os animais foram divididos em controle e suplementados com leucina (1,35 g/kg por dia), a qual teve início três dias antes da criolesão e prosseguiu por um, três, 10 e 21 dias. A leucina aumentou a AST das fibras no soleus e reduziu o processo inflamatório e a fibrose em ambos, além de reduzir a ativação de elementos da via TGF-β/Smad. A leucina também aumentou o número de células satélites em proliferação no soleus e acelerou a conversão das isoformas de MyHC em ambos, tendo aumentado a taxa de síntese proteica no TA. Entretanto, não houve elevação na expressão de elementos da via PI3K/Akt/mTOR. Observou-se redução da ativação de FoXO3a e do acúmulo de proteínas ubiquitinadas no soleus, além de maior atividade do proteassomo 26S. A leucina também atenuou o prejuízo funcional em ambos.
Title in English
Effect of leucine supplementation on skeletal muscle regenerative process.
Keywords in English
Leucine supplementation
Regeneration
Skeletal muscle damage
Abstract in English
Leucine supplementation can be an important therapy for the regeneration of injured skeletal muscle tissue, for modulating intracellular pathways regulating muscle mass. In this study, rats had their soleus and TA muscles of the left hind limb damaged. The animals were divided into control and supplemented with leucine groups (1.35 g / kg per day), which started three days before damage and continued for 1, 3, 10 and 21 days. Leucine increased myofiber CSA in soleus and reduced inflammation and fibrosis on both, and reduced the activation of TGF-β/Smad signaling pathways. Leucine also increased the number of proliferating satellite cells in the soleus, accelerated the conversion between MyHC isoforms, and in rate of protein synthesis in the TA. However, no elevation on PI3K/Akt/mTOR signalling pathway was observed. There was a reduction on FoXO3a activation and ubiquitinated proteins accumulation in the soleus and an increase in the 26S proteasome activity. Leucine also attenuated the functional impairment on both muscles.
 
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Release Date
2017-08-26
Publishing Date
2015-08-28
 
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