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Doctoral Thesis
DOI
10.11606/T.42.2014.tde-14112014-154208
Document
Author
Full name
Meiricris Tomaz da Silva
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2014
Supervisor
Committee
Miyabara, Elen Haruka (President)
Bacurau, Reury Frank Pereira
Ferreira, Cecilia Helena de Azevedo Gouveia
Freitas, Vanessa Morais
Marques, Maria Julia
Title in Portuguese
Papel do adrenoceptor beta 2 na regeneração muscular esquelética.
Keywords in Portuguese
Adrenoceptor b2
Akirina1
Contração muscular
Macrófagos
Regeneração muscular esquelética
Smad
Abstract in Portuguese
No intuito de avaliar o papel do receptor b2-adrenérgico no processo de regeneração muscular, os músculos tibialis anterior de camundongos knockout para o adrenoceptor b2 (b2KO) foram criolesados e analisados após 1, 3, 10 e 21 dias. Análises de aspectos morfológicos e contráteis, atuação de macrófagos M1 e M2, conteúdo de AMPc e ativação de elementos da via de sinalização TGF-b/smad foram realizadas. Os músculos em regeneração dos animais b2KO apresentaram redução do calibre das fibras musculares, redução na função contrátil em 10 dias após criolesão, atenuado aumento do conteúdo de AMPc nos músculos em 10 dias após criolesão, aumento da inflamação e do número de macrófagos nos músculos em regeneração em 3 e 10 dias após lesão, predominância de macrófagos M1, diminuição da ativação de TbR-I e smad2/3 e da expressão de smad4 em 3 dias após lesão, e aumento na expressão de akirina1 em 10 dias após lesão. Nossos resultados sugerem que o adrenoceptor b2 contribui para a regulação das fases iniciais da regeneração muscular.
Title in English
The role of beta 2 adrenoceptor in skeletal muscle regeneration.
Keywords in English
b2-adrenoceptor
Akirin1
Contraction
Macrophage
Skeletal muscle regeneration
Smad
Abstract in English
In this study, we investigated the role of the b2-adrenoceptor in skeletal muscle regeneration. Tibialis anterior muscles from b2-adrenoceptor knockout (b2KO) mice were cryolesioned and analysed after 1, 3, 10, and 21 days. Analysis of structural and contractile aspects, M1 and M2 macrophage profile, cAMP content, and activation of TGF-b/smad signalling elements. Regenerating muscles from b2KO mice showed diminished calibre of regenerating myofibres, decreased muscle contractile function at 10 days when compared with those from wild-type, attenuated augment in cAMP content in muscles at 10 days post-injury, increase in inflammatory process and in the number of macrophages at 3 and 10 days, prevalence of M1 macrophage phenotype, reduction in TbR-I and smad2/3 activation, and in the smad4 expression at 3 days, and an increase in akirin1 expression at 10 days in muscles from b2KO mice when compared to those from wild-type. Our data suggest that the b2-adrenoceptor contributes to the control of the initial stages of muscle regeneration.
 
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Release Date
2016-11-26
Publishing Date
2014-11-27
 
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