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Master's Dissertation
DOI
10.11606/D.42.2014.tde-13112014-160412
Document
Author
Full name
Amanda Teixeira Rodrigues
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2014
Supervisor
Committee
Lotfi, Claudimara Ferini Pacicco (President)
Almeida, Madson Queiroz de
Freitas, Vanessa Morais
Title in Portuguese
Caracterização molecular e funcional de células de tumores adrenocorticais humanos.
Keywords in Portuguese
Culturas de células
Esteroidogênese
Expressão gênica
Genes supressores de tumor
Oncogenes
Tumor adrenocortical
Abstract in Portuguese
O Adenoma adrenocortical é frequente em adultos, já o carcinoma é raro e agressivo. Mesmo com critérios padronizados, ainda há dificuldade para diferenciar esses tumores, sendo necessário o estudo de marcadores eficientes na detecção e diferenciação. Por serem raros e com diversas manifestações clínicas, culturas in vitro pode ser uma ferramenta para o estudo de processos que envolvem a doença. Foi realizada a caracterização molecular e funcional de culturas de células de tumores de pacientes. Resultados de PCR Array não mostraram um padrão que diferenciasse as culturas em função dos diagnósticos. Desta análise, 7 oncogenes apresentaram maior expressão e 9 supressores de tumor apresentaram baixa expressão nas culturas. WWOX, FHIT e TP73 foram validados por qPCR e a sugestiva interação entre esses fatores nos tumores adrenocorticais merecem futuras investigações. O potencial funcional das culturas T83-ACC, T36-REC e T7-ACA(P) foram evidenciados, e mostraram que podem ser bons modelos para estudo da ação de hormônios e seus mecanismos.
Title in English
Molecular and functional characterization of human adrenocortical cell cultures.
Keywords in English
Adrenocortical tumor
Cell cultures
Gene expression
Oncogenes
Steroidogenesis
Tumor suppressor genes
Abstract in English
The adrenocortical adenoma is common in adults, since carcinoma is rare and aggressive. Even with standardized scores, it is still difficult to differentiate these tumors, the study of efficient markers in the detection and differentiation is necessary. Because they are rare and diverse clinical manifestations in vitro cultures can be a tool for the study of disease processes that involve. Molecular and functional characterization of cultured tumor cells of patients was conducted. PCR Array results did not show a pattern that differentiates cultures on the basis of diagnoses. This analysis showed higher expression 7 oncogenes and tumor suppressors 9 showed low expression in cultures. WWOX, FHIT and TP73 were validated by qPCR and suggestive interaction between these factors in adrenocortical tumors deserve further investigation. The functional potential of T83-ACC, T36-REC and T7-ACA(P) cell cultures were found, and shown confirm that they can be good models for studying the action of hormones and their mechanisms.
 
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Release Date
2016-11-26
Publishing Date
2014-11-27
 
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