• JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
 
  Bookmark and Share
 
 
Doctoral Thesis
DOI
https://doi.org/10.11606/T.42.2016.tde-09112016-133400
Document
Author
Full name
João Guilherme de Oliveira Silvestre
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2016
Supervisor
Committee
Moriscot, Anselmo Sigari (President)
Ferrari, Raquel Agnelli Mesquita
Lotfi, Claudimara Ferini Pacicco
Nunes, Maria Tereza
Zanoteli, Edmar
 
Title in Portuguese
Papel do MuRF1 e MuRF2 sobre aspectos estruturais e funcionais de mioblastos e fibroblastos musculares esqueléticos.
Keywords in Portuguese
E3 ligases
Fibroblastos
Mioblastos
Músculo esquelético
Abstract in Portuguese
As E3 ligases MuRF1 e MuRF2 tem sido descritas com importantes papéis na estabilidade de proteínas da estrutura muscular, além de contribuírem para marcação de proteínas que devem ser degradadas. Nosso objetivo foi verificar o papel de MuRF1 e MuRF2 na diferenciação de células miogênicas, além de caracterizar seu papel em fibroblastos. Foram utilizados animais nocautes para MuRF1 e MuRF2 e verificamos o processo de regeneração 28 dias após a injeção de cardiotoxina no tibial anterior. Posteriormente, através de análises in vitro, realizamos o silenciamento de MuRF1 e MuRF2 utilizando RNAis e verificamos a capacidade de diferenciação de células miogênicas. Os resultados mostram que os animais nocautes apresentaram aumento de marcadores adipogênicos. Além disso, as células silenciadas com RNAis apresentaram queda na formação de miotubos e um aumento em marcadores adipogênicos. Em fibroblastos, identificamos as E3 ligases MuRF1 e MuRF2 e o RNAi nessas células prejudicou o processo de migração. Esses resultados realçam a importância de MuRF1 e MuRF2 na diferenciação miogênica além de sugerir um importante papel para a formação correta do citoesqueleto durante a migração celular.
 
Title in English
Role of MuRF1 and MuRF2 on functional and structural aspects of myoblasts and fibroblasts skeletal muscle cells.
Keywords in English
E3 ligases
Fibroblasts
Myoblasts
Skeletal muscle
Abstract in English
The E3 ligases MuRF1 and MuRF2 has been proposed to act as a linker for myofibril machinery, also by acting as an Atrogene during muscle wasting. Our aim was to verify the role of MuRF1 and MuRF2 during the myogenic differentiation of skeletal muscle and its role on skeletal muscle fibroblasts. We used MuRF1 and MuRF2 knockout mice and analyzed the regenerative process. Using in vitro analyzes, we silenced MuRF1 and MuRF2 expression by siRNA. Our results suggest that knockout mice had an important impairment on skeletal muscle regeneration, showing positive staining to white adipocytes. Moreover, siRNA on myogenic primary cultures showed impaired myotube formation and increase the expression of adipogenic markers. Another interesting finding was that skeletal muscle fibroblasts can express MuRF1 and MuRF2, and its silencing by siRNA impairs the migration capacity of fibroblasts. These results demonstrate the importance of MuRF1 and MuRF2 during myogenic differentiation of skeletal muscle and an important role at intracellular coordination of stress fiber formation of skeletal muscle fibroblasts.
 
WARNING - Viewing this document is conditioned on your acceptance of the following terms of use:
This document is only for private use for research and teaching activities. Reproduction for commercial use is forbidden. This rights cover the whole data about this document as well as its contents. Any uses or copies of this document in whole or in part must include the author's name.
Publishing Date
2016-11-09
 
WARNING: Learn what derived works are clicking here.
All rights of the thesis/dissertation are from the authors.
CeTI-SC/STI
© 2001-2024. Digital Library of Theses and Dissertations of USP.