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Master's Dissertation
DOI
https://doi.org/10.11606/D.42.2008.tde-08092008-122845
Document
Author
Full name
Luciano Gonçalves
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2008
Supervisor
Committee
Lagnado, Sara Joyce Shammah
Metzger, Martin Andreas
Toledo, Claudio Antonio Barbosa de
Title in Portuguese
Organização das projeções do córtex pré-fontral para o núcleo dorsal da rafe no rato.
Keywords in Portuguese
Córtex pré-frontal
CTb
Imunoistoquímica
Núcleo dorsal da Rafe
Rato
Traçador neuronal
Abstract in Portuguese
O núcleo dorsal da rafe (DR) envia densas projeções para o prosencéfalo e está implicado em várias funções complexas. Muitos estudos indicam que a atividade neuronal do DR é controlada por neurônios do córtex pré-frontal (PFC). Devido à escassez de informações detalhadas sobre as conexões entre o PFC e o DR, objetivamos mapear sistematicamente as projeções do PFC para o DR. O traçador neuronal toxina colérica subunidade b (CTb) foi injetado nas diferentes partes do DR. Com técnica imunoistoquímica contra a CTb, células CTb-ir foram encontradas em maior escala no córtex polar frontal e na parede medial do PFC. No PFC lateral, o córtex da insula agranular dorsal, apresentou maior densidade de neurônios CTb-ir. No PFC orbital, o córtex orbital lateral e dorso-lateral eferentam principalmente a parte central do DR. Injeções situadas na parte caudal do DR resultaram em marcação atenuada. Os resultados indicam que o PFC envia robustas projeções para o DR de forma diferenciada. Através dessas projeções o PFC pode exercer um controle "top down" sobre os neurônios do DR.
Title in English
Organization of the projections from the prefrontal cortex to the dorsal raphe nucleus in the rat.
Keywords in English
CTb
Dorsal Raphe nucleus
Immunohistochemistry
Neuronal tracing
Prefrontal cortex
Rat
Abstract in English
The dorsal raphe nucleus (DR) sends massive projections to the forebrain and is implicated in a variety of complex functions. Several studies indicate that neuronal activity in the DR is controlled by the prefrontal cortex (PFC). Since there is no detailed information about the projections from the PFC to the DR, the goal of the present study was to map these projections systematically. The neuronal tracer cholera toxin, subunit b (CTb) was injected at different levels of the DR. By immunohistochemical methods, CTb-ir cells were found highly enriched in the frontal polar cortex and in the medial wall of the PFC. In the lateral PFC, the dorsal lateral insular cortex presented the highest density of CTb-ir neurons. In the orbital PFC, the lateral and dorso-lateral orbital cortex project principally to the central segment of the DR. Injections in the caudal segment of the DR resulted in reduced CTb staining. Our results indicate that the PFC sends massive topographically organized projections to the DR, which may exert a "top down" control over neurons in the DR.
 
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Publishing Date
2008-09-10
 
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