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Master's Dissertation
DOI
https://doi.org/10.11606/D.25.2022.tde-25012023-184430
Document
Author
Full name
Caíque Andrade Santos
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
Bauru, 2022
Supervisor
Committee
Zangrando, Mariana Schutzer Ragghianti (President)
Damante, Carla Andreotti
Teodoro, Guilherme Rodrigues
Vilhena, Fabiano Vieira
Title in Portuguese
O derivado de ftalocianina é menos citotóxico e não influencia negativamente a cicatrização in vitro de feridas comparado à clorexidina
Keywords in Portuguese
Agente fotossensibilizante
Cicatrização
Clorexidina
Abstract in Portuguese
O uso de substâncias químicas auxiliares no pós-operatório precoce de procedimentos cirúrgicos periodontais é recomendado, uma vez que nesse período o paciente apresenta dor e desconforto e a remoção mecânica de biofilme tem potencial risco de trauma na área operada. O uso de enxaguatório com digluconato de clorexidina (CHX) é considerado padrão ouro no controle químico do biofilme supragengival. Contudo, seu uso pode causar distúrbio e alteração no paladar, ulceração na mucosa e manchamento de dentes; além disso, estudos laboratoriais evidenciam grande citotoxicidade do composto. Os derivados de ftalocianinas (PHY) vêm sendo amplamente estudados em literatura devido seus efeitos positivos associado a Terapia Fotodinâmica Antimicrobiana (aTFD), apresentando, sobretudo, boa atividade antimicrobiana. Uma classe ainda pouco explorada de PHY é a autoativada, caracterizada por um amplo espectro e ativação na ausência de luz, produtos químicos ou eletricidade, exceto oxigênio molecular. Sendo assim, o objetivo do estudo foi comparar a citotoxicidade in vitro de CHX e PHY e avaliar a influência dos compostos na cicatrização experimental. Diferentes concentrações de CHX e PHY (0,0075% - 0,12%) permaneceram em contato por um minuto com fibroblastos NIH 3T3 sendo avaliada a viabilidade celular pelo ensaio de MTT e cristal violeta (CV); CHX e PHY (0,0075% e 0,12%) também foram avaliadas no ensaio de cicatrização in vitro. A PHY apresentou-se menos citotóxica comparada a CHX, a partir dos ensaios de viabilidade celular. A PHY não interferiu na cicatrização experimental, permitindo a migração celular semelhante ao controle positivo nas duas concentrações estudadas de PHY 0,0075% e PHY 0,12%, a medida em que apenas CHX 0,0075% permitiu a migração de células. Em uma análise comparativa, PHY revelou menor citotoxicidade que CHX, sendo as concentrações de PHY 0,0075% e 0,015% atóxicas mesmo em 48 horas de contato com as células. Podemos concluir que a PHY se apresentou menos citotóxica para fibroblastos NIH 3T3 comparado a CHX, através de dois ensaios para avaliação da viabilidade celular (MTT e CV). Além disso, as diferentes concentrações de PHY não interferiram negativamente na cicatrização de feridas experimentais. Já a CHX permitiu o fechamento apenas em concentrações menores.
Title in English
The phthalocyanine derivative is less cytotoxic and does not negatively influence in vitro wound healing compared to chlorhexidine
Keywords in English
Chlorhexidine
Photosensitizing agents
Wound Healing
Abstract in English
The use of auxiliary chemical substances in the early postoperative period of periodontal surgical procedures is recommended, since during this period the patient presents pain and discomfort and the mechanical removal of biofilm has a potential risk of trauma in the operated area. The use of chlorhexidine digluconate (CHX) mouthwash is considered the gold standard in the chemical control of supragingival biofilm. However, its use can cause disturbance and alteration in taste, ulceration in the mucosa and staining of teeth; in addition, laboratory studies show great cytotoxicity of the compound. Phthalocyanine derivatives (PHY) have been widely studied in the literature due to their positive effects associated with Antimicrobial Photodynamic Therapy (aPDT), showing, above all, good antimicrobial activity. A still little explored class of PHY is the self-activated one, characterized by a broad spectrum and activation in the absence of light, chemicals or electricity, except molecular oxygen. Therefore, the aim of the study was to compare the in vitro cytotoxicity of CHX and PHY and to evaluate the influence of the compounds on experimental healing. Different concentrations of CHX and PHY (0,0075% - 0,12%) remained in contact for one minute with NIH 3T3 fibroblasts, and cell viability was evaluated by the MTT and crystal violet (CV) assay; CHX and PHY (0,0075% and 0,12%) were also evaluated in the in vitro healing assay. PHY was less cytotoxic compared to CHX, based on cell viability assays. PHY did not interfere with experimental healing, allowing cell migration similar to the positive control at the two concentrations studied of PHY 0,0075% and 0,12% PHY, as only 0,0075% CHX allowed cell migration. In a comparative analysis, PHY showed less cytotoxicity than CHX, with PHY concentrations of 0,0075% and 0,015% being non-toxic even after 48 hours of contact with the cells. We can conclude that PHY was less cytotoxic to NIH 3T3 fibroblasts compared to CHX, through two assays to evaluate cell viability (MTT and CV). Furthermore, the different concentrations of PHY did not interfere negatively in the healing of experimental wounds. CHX, on the other hand, allowed closure only at lower concentrations.
 
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Publishing Date
2023-02-07
 
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