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Master's Dissertation
DOI
10.11606/D.25.2014.tde-17072014-094006
Document
Author
Full name
Francisco Barbara Abreu Barros
Institute/School/College
Knowledge Area
Date of Defense
Published
Bauru, 2014
Supervisor
Committee
Oliveira, Denise Tostes (President)
Bernabé, Daniel Galera
Gasparoto, Thais Helena
Title in Portuguese
Expressão de moesina e podoplanina no câncer de boca e sua relação com o processo de invasão tumoral
Keywords in Portuguese
Biomarcadores Farmacológicos
Câncer de boca
Carcinoma de células escamosas
Abstract in Portuguese
A proteína moesina, uma das proteínas do complexo ERM (ezrina, radixina e moesina), participa do processo de migração de células tumorais controlando a ligação entre o citoesqueleto de actina e os receptores transmembrana. As proteínas ERM vêm sendo investigadas como ligantes de outras glicoproteínas, como a podoplanina, cuja expressão é encontrada em células malignas de diversas neoplasias, incluindo o carcinoma espinocelular (CEC) de boca. O objetivo desse estudo foi avaliar as expressões imuno-histoquímicas da moesina e da podoplanina pelas células malignas no front de invasão de 84 pacientes com CEC de boca e suas associações com a evolução clínica e com o prognóstico dos pacientes. A associação entre a expressão imuno-histoquímica da moesina e da podoplanina pelas células malignas e as variáveis demográficas, clínicas e microscópicas foi avaliada pelo teste qui-quadrado ou teste exato de Fisher. As análises de sobrevida global e livre de doença em 5 e 10 anos foram calculadas pelo estimulador produto-limite de Kaplan-Meier e a comparação das curvas de sobrevida realizada pelo teste de log-rank. Os resultados mostraram que houve expressão da moesina pelas células malignas na região do front de invasão tumoral, entretanto, nenhuma associação estatisticamente significativa foi encontrada entre esta proteína e as características clínicas, demográficas e microscópicas. A expressão da podoplanina, pelas células malignas, foi significativamente associada à radioterapia (p=0,004), à invasão muscular (p=0,006) e ao comprometimento linfonodal (p=0,013). Não houve associação significativa entre a expressão das duas proteínas nos CECs de boca (p=0,460). A forte expressão da moesina pelas células malignas constituiu um fator de prognóstico desfavorável para os pacientes com CEC de boca e estadiamento clínico II e III. O comprometimento linfonodal histopatológico também se mostrou fator de prognóstico significativo para a recidiva da doença (p=0,018). Estes resultados sugerem que a expressão de moesina, pelas células malignas juntamente com o comprometimento linfonodal pode contribuir para determinar os pacientes com CEC de boca que apresentam um pior prognóstico. Além disso, verificamos que as proteínas moesina e podoplanina se expressam pelas células neoplásicas nos CEC de boca mas não parecem estar associadas no processo de invasão tumoral.
Title in English
Expression of moesin and podoplanin in oral cancer and its relation to the process of tumor invasion
Keywords in English
Oral cancer
Pharmacological Biomarkers
Squamous Cell Carcinoma
Abstract in English
The moesin protein, one of the proteins of the ERM complex (ezrin, radixin and moesin) takes part in the migration of tumor cells process by controlling the relation between actin cytoskeleton and transmembrane receptors. The ERM proteins have been investigated as ligants of other glycoproteins, such as podoplanin, which are found in malignant cells of malignant, including oral squamous cell carcinoma (OSCC). The aim of this study was to evaluate the immunohistochemical expressions of moesin and podoplanin by malignant cells in the invasive front of 84 patients with oral squamous carcinoma and its association with clinical outcome and patients' prognosis. Chi- square or Fisher's exact test was used to analyze the association between the moesin and podoplanin expressions by malignant cells and demographic, clinical and microscopic variables in oral squamous cell carcinoma patients. The 5 and 10 years survival rates were calculated by Kaplan-Meier method and the comparison of survival curves were performed using log-rank test. The results showed that there was moesin expression by malignant cells in the invasive front, however, no statistically significant association was found between this protein and demographic, clinical and microscopic features. The expression of podoplanin by malignant cells was significantly associated with radiotherapy (p=0.004), with muscular invasion (p=0.006) and lymph node involvement (p=0.013). There was no significant association between the expression of two proteins in OSCC (p=0.460). The strong expression of moesin by malignant cells was a factor of unfavorable prognosis for patients with OSCC and clinical stage II and III. The histopathological lymph node involvement was also significant prognostic factor for disease recurrence (p=0.018). These results suggest that the expression of moesin by malignant cells and lymph node involvement may help to determine patients with squamous cell carcinoma who have a poor prognosis. Furthermore, we found that moesin and podoplanin proteins are expressed by neoplastic cells in oral squamous cell carcinoma but not appear to be associated in the process of tumor invasion.
 
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Publishing Date
2014-07-17
 
WARNING: The material described below relates to works resulting from this thesis or dissertation. The contents of these works are the author's responsibility.
  • ASSAO, Agnes, et al. Displacement of dental implant into the focal osteoporotic bone marrow defect. In XXI Congresso Brasileiro de Estomatologia e Patologia Oral., Salvador, BA, 2014. Oral Surg Oral Med Oral Pathol Oral Radiol., 2014. Abstract.
  • Barros, FBA, et al. Lesión radiolúcida en el ángulo de la mandíbula: importancia del diagnóstico diferencial. In X Reunión Internacional de la Academia Iberoamericana de Patologia y Medicina Bucal y IV Congreso Internacional de Patologia y Cirurgia Bucomaxilofacial., Cartagena, 2012. Medicina Oral, Patología Oral y Cirugía Bucal (Ed. impresa)., 2012. Resumo.
  • Barros, FBA, et al. Manifestação bucal da sífilis secundária em paciente idoso. In XXV Congresso Odontológico de Bauru, Bauru, 2012. XXV Congresso Odontológico de Bauru - USP., 2012. Resumo.
  • Barros, FBA, et al. Mucoepidermoid carcinoma with extensive mandibular destruction. In XXI Congresso Brasileiro de Estomatologia e Patologia Oral., Salvador, BA, 2014. Oral Surg Oral Med Oral Pathol Oral Radiol., 2014. Abstract.
  • Barros, FBA, et al. Multiple fibro-osseous lesions of the mandible. In XXI Congresso Brasileiro de Estomatologia e Patologia Oral., Salvador, BA, 2014. Oral Surg Oral Med Oral Pathol Oral Radiol., 2014. Abstract.
  • Barros, FBA, et al. Multiple keratocystic odontogenic tumor - case report. In 16th International Congress on Oral Pathology and Medicine and 20th Brazilian Congress of Oral Medicine and Pathology 2012 (São Pedro-Brazil, 30th July 03rd August 2012), São Pedro, 2012. Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology., 2012. Abstract.
  • Garcia, NG, et al. Angiosarcoma of the maxilla: case report. In XXI Congresso Brasileiro de Estomatologia e Patologia Oral., Salvador, BA, 2014. Oral Surg Oral Med Oral Pathol Oral Radiol., 2014. Abstract.
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