• JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
 
  Bookmark and Share
 
 
Master's Dissertation
DOI
10.11606/D.25.2011.tde-09122011-094949
Document
Author
Full name
Adriana dos Santos Caetano
Institute/School/College
Knowledge Area
Date of Defense
Published
Bauru, 2011
Supervisor
Committee
Oliveira, Denise Tostes (President)
Matsumoto, Mariza Akemi
Rosa, Luiz Eduardo Blumer
Title in Portuguese
Imunolocalização da podoplanina em tumores odontogênicos benignos
Keywords in Portuguese
Podoplanina
Tumores benignos
Tumores odontogênicos
Abstract in Portuguese
A podoplanina humana é uma glicoproteína que se expressa em várias células e tecidos normais e neoplásicos, inclusive aqueles de origem odontogênica. O objetivo deste estudo foi identificar a imunolocalização da podoplanina em tumores odontogênicos epiteliais com e sem ectomesênquima incluindo oito ameloblastomas, nove tumores odontogênicos adenomatóides, vinte tumores odontogênicos queratocísticos, cinco cistos odontogênicos ortoqueratinizados, um tumor odontogênico epitelial calcificante, dois fibromas ameloblásticos, quatro fibroodontomas ameloblásticos e cinco tumores odontogênicos císticos calcificantes. Todos os tumores odontogênicos foram submetidos a imuno-histoquímica para o anticorpo anti-podoplanina numa diluição de 1:100 e avaliados, microscopicamente, com base na distribuição tecidual e na intensidade da imunomarcação. Para os tumores odontogênicos queratocísticos e cistos odontogênicos ortoqueratinizados além da podoplanina foi determinado o índice de proliferação celular baseado na positividade nuclear das células do epitélio odontogênico imunomarcadas com o Ki-67 na diluição de 1:200 e comparados estatisticamente pelo coeficiente de correlação de Spearman. Os resultados mostraram uma forte expressão da podoplanina na membrana e no citoplasma do epitélio odontogênico da maioria dos tumores analisados, bem como, em células ectomesênquimais como os odontoblastos e suas extensões dentinárias. A ausência da podoplanina foi identificada nos ameloblastos completamente diferenciados, nas áreas de metaplasia escamosa, nas células fantasmas, nas áreas de calcificação e nos depósitos extracelulares de material eosinofílicos observados nos tumores odontogênicos. No tumor odontogênico queratocístico observou-se uma forte expressão da podoplanina na camada basal e suprabasal do epitélio, enquanto que, nos cistos odontogênicos ortoqueratinizados esta expressão estava ausente ou fracamente distribuída no epitélio. Houve uma correlação estatisticamente significativa (p=0,006) entre a expressão de podoplanina e o índice de proliferação celular dos tumores odontogênicos queratocísticos e cistos odontogênicos ortoqueratinizados. Estes resultados sugerem que a podoplanina participa dos processos de proliferação e diferenciação celular dos epitélios odontogênicos presentes nos tumores odontogênicos benignos dos ossos maxilares.
Title in English
Immunolocalization of the podoplanin in benign odontogenic tumours
Keywords in English
Benign tumor
Odontogenic tumor
Podoplanin
Abstract in English
Human podoplanin is a glycoprotein expressed in various cells and normal and neoplastic tissues, including those of odontogenic origin. The aim of this study was to identify the immunolocalization of podoplanin in epithelial odontogenic tumors with and without ectomesenchyme, including eight ameloblastomas, nine adenomatoid odontogenic tumors, twenty keratocystic odontogenic tumors, five orthokeratinized odontogenic cysts, one calcifying epithelial odontogenic tumor, two ameloblastic fibromas, four ameloblastic fibro-odontomas and five calcifying cystic odontogenic tumors. All odontogenic tumors were submitted to immunohistochemistry using a podoplanin antibody at a dilution of 1:100 and evaluated microscopically, based on the tissue distribution and intensity of immunoreactivity. For keratocystic odontogenic tumors and orthokeratinized odontogenic cysts, in addition to podoplanin, the index of cell proliferation was determined based on the nuclear positivity of odontogenic epithelial cells immunostained with Ki-67 at a dilution of 1:200 and statistically compared by the Spearman correlation coefficient. The results showed strong expression of podoplanin in the membrane and cytoplasm of the odontogenic epithelium of most tumors analyzed, as well as in ectomesenchymal cells as odontoblasts and dentinal projections. Absence of podoplanin was observed in fully differentiated ameloblasts, in areas of squamous metaplasia, in ghost cells, in areas of calcification and extracellular deposits of eosinophilic material observed in odontogenic tumors. The keratocystic odontogenic tumor exhibited strong expression of podoplanin in basal and suprabasal epithelial layers, while in orthokeratinized odontogenic cysts this expression was absent or weakly distributed in the epithelium. There was statistically significant correlation (p=0,006) between the expression of podoplanin and the cellular proliferation index of odontogenic tumors and orthokeratinized odontogenic cysts. These results suggest that podoplanin participates in the processes of cell proliferation and differentiation of odontogenic epithelium present in benign odontogenic tumors of the jaws.
 
WARNING - Viewing this document is conditioned on your acceptance of the following terms of use:
This document is only for private use for research and teaching activities. Reproduction for commercial use is forbidden. This rights cover the whole data about this document as well as its contents. Any uses or copies of this document in whole or in part must include the author's name.
Publishing Date
2011-12-09
 
WARNING: The material described below relates to works resulting from this thesis or dissertation. The contents of these works are the author's responsibility.
  • Caetano, AS, et al. Immunolocalization of podoplanin in benign odontogenic tumors with and without ectomesenchyme [doi:10.1016/j.archoralbio.2012.06.002]. Archives of Oral Biology [online], 2013, vol. 58, p. 408-415.
  • Caetano, AS, et al. Immunolocalization of podoplanin in benign odontogenic tumors [doi:10.1111/j.1365-2559.2010.03637.x]. In XXVIIIth International Congress of the International Academy of Pathology., São Paulo, 2010. Histopathology., 2010. Abstract.
  • Carvalho, C.A.P., et al. Imunoexpresión de podoplanina en células epiteliales odontogênicas tumorales. In XI Congreso de la Sociedad Española de Medicina Oral y IX Reunión de la Academia Iberoamericana de Patologia y Medicina Bucal., Córdoba - Espanha, 2011. Medicina Oral, Patología Oral y Cirugía Bucal (Ed. impresa)., 2011. Resumo.
  • TJIOE, Kellen Cristine, et al. Expresión de podoplanina en tumores odontogênicos epiteliales. In XLIII Reunión Anual de la Sociedad Argentina de Investigación Odontológica - División Argentina de la International Association for Dental Research., Los Cocos, 2010. Anais de la XLIII Reunión Anual de la Sociedad Argentina de Investigación Odontológica., 2010. Resumo.
  • TJIOE, Kellen Cristine, et al. Expression of podoplanin in epithelial odontogenic tumors. In XLIII Reunión Anual de la Sociedad Argentina de Investigación Odontologica División Argentina de la International Association for Dental Research, Los Cocos, 2010. Journal of Dental Research., 2010. Abstract.
  • TJIOE, Kellen Cristine, et al. Imunolocalização da podoplanina e seu papel nos tumores odontogênicos epiteliais benignos. In XXVIII Reunião Anual da Sociedade Brasileira de Pesquisa Odontológica - Brazilian Division of the International Association for Dental Research., Águas de Lindóia, 2011. Brazilian Oral Research ., 2011. Resumo.
All rights of the thesis/dissertation are from the authors
Centro de Informática de São Carlos
Digital Library of Theses and Dissertations of USP. Copyright © 2001-2021. All rights reserved.