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Master's Dissertation
DOI
10.11606/D.25.2014.tde-17072014-150102
Document
Author
Full name
Tamiris Vallim Zambaldi de Oliveira
Institute/School/College
Knowledge Area
Date of Defense
Published
Bauru, 2014
Supervisor
Committee
Assis, Gerson Francisco de (President)
Cestari, Tânia Mary
Hassunuma, Renato Massaharu
Title in Portuguese
Avaliação histomorfométrica da biocompatibilidade do enxerto bovino misto (OrthoGen®) em tecido subcutâneo e o potencial osteogênico em defeito ósseo craniano
Keywords in Portuguese
Materiais biocompatíveis
Ratos
Regeneração óssea
Teste de materiais
Abstract in Portuguese
O desenvolvimento atual de materiais ósseo-substitutos com potencialidade de promover o fechamento completo de um defeito ósseo crítico tem levantado questões quanto à sua atuação biológica. Uma opção de material ósseo utilizado são os enxertos ósseos de origem animal, pois possuem propriedades físicoquímicas similares ao osso humano. O osso bovino misto que preserva a estrutura colagênica e o mineral ósseo tem sido proposto e utilizado como material ósseosubstituto. O objetivo desse estudo foi avaliar a biocompatibilidade e o potencial osteogênico de um enxerto bovino ósseo subistituto, OrthoGen® (Baumer S.A.), na forma de partículas (OGp) e blocos (OGb). Para a avaliação da biocompatibilidade, 100 mg de OrthoGen® nas formas de partícula e bloco, foram implantados no subcutâneo de ratos (n=25) e o tecido reacional foi avaliado aos 7, 14, 21, 30 e 60 dias (n=5 animais/período) após a implantação. Para a análise do potencial osteogênico foram implantados 100 mg de Orthogen® nas formas de partícula e bloco em defeito critico na calvária de ratos (n=30), e a formação óssea foi mensurada aos 1, 3 e 6 meses (n=10/período) após a implantação. A análise radiográfica e histomorfométrica revelaram que no tecido subcutâneo o OGb foi melhor aceito pelo organismo quando comparado ao OGp com uma frequência menor de células gigante multinucleadas entre os períodos avaliados (OGb 0,23% vs OGp 2,19%) e consequentemente uma média de reabsorção também menor (OGb 13% vs OGp 38%). Em ambos os implantes não foi encontrado focos de infiltrado inflamatório composto por leucócitos polimorfonucleares, linfócitos e plasmócitos. Na calvária o OGb mostrou níveis de reabsorção inferiores e uma maior taxa de formação óssea quando comparado ao OGp após 6 meses (OGb 70 mm³ vs OGp 17 mm³). Baseado no modelo experimental utilizado neste estudo, concluímos que ambas as formas do Orthogen® são biocompatíveis em tecido subcutâneo, no entanto, sua forma em bloco promove uma maior formação óssea, possuindo uma capacidade osteogênica superior à forma em partícula, no modelo experimental avaliado.
Title in English
Histomorphometric evaluation of biocompatibility of integral bovine graft (Orthogen®) implanted in subcutaneous tissue and osteogenic potential in cranial bone defect
Keywords in English
Biocompatible materials
Bone regeneration
Materials testing
Rats
Abstract in English
The current development of bone graft materials with the potential to promote the complete closure of a critical size bone defect has raised questions as to its biological activity. An option of bone material used, are animal bone grafts since the human bone have similar physicochemical properties. Among the materials is the integral bone substitute of bovine origin, which preserves the organic and inorganic compound of the bone tissue, has been proposed and used as bone graft. The aim of this study was to evaluate the biocompatibility and osteogenic potential of a new integral bone substitute OrthogenTM (Baumer S.A.) in the form of particles (OGp) and block (OGb). For biocompatibility evaluation, 100mg OrthogenTM was implanted into dorsal subcutaneous pocket of rat (n= 25) and the reactional tissue was analyzed at 7, 14, 21, 30 and 60 days (n=5animals/period) after implantation. For osteogenic potential evaluation, 100mg OrthogenTM was implanted into critical-size defect in parietal bones of rat (n=30) and the bone formation, biomaterial reabsorption, connective tissue formation and osteoclast activity was evaluated at 1, 3 and 6 months (n=10/period) after implantation. Radiographic and histomorphometrical analysis showed that, in the subcutaneous tissue the OGb was more accepted by the host compared to OGp, with lower density of the multinucleated giant cells (OGb 0.23% vs. OGp 2.19%) and consequently a lower rate of matrix resorption (OGb 13%, vs. OGp 38%). In both implants was not found focus of inflammatory infiltrated composed by polymorphonuclear leucocytes, lymphocytes and plasmocytes. In rat calvaria the OGb showed lower rate of reabsorption and more volume of bone formation compared to OGp after 6 months (OGb 70 mm3 vs OGp 17 mm3). Based on experimental models used in this study we concluded that both forms of the OrthogenTM was biocompatible in subcutaneous tissue, however, its form of porous block promoted greater bone formation and has a higher osteogenic capacity than the particle shape, in the evaluated experimental model.
 
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Publishing Date
2014-07-17
 
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