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Master's Dissertation
DOI
10.11606/D.23.2009.tde-19122009-111607
Document
Author
Full name
Arthur Rodriguez Gonzalez Côrtes
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2009
Supervisor
Committee
Sousa, Suzana Cantanhede Orsini Machado de (President)
Pinto Júnior, Décio dos Santos
Siqueira, Filipe Modolo
Title in Portuguese
Comparação imunoistoquímica das expressões das proteínas p27 e c-jun na carcinogênese intra-oral
Keywords in Portuguese
Carcinoma epidermóide
Displasia epitelial
Imunoistoquímica
p27 c-jun
Abstract in Portuguese
A expressão de diversas proteínas do ciclo celular tem sido estudada em vários tipos de lesões malignas. Entre as principais está a p27, que é codificada pelo gene supressor de tumor de mesmo nome, e que já foi encontrada alterada em carcinomas, inclusive os intra-orais. Outra proteína que atua diretamente no controle do ciclo celular é a c-jun, que é codificada por um protooncogene, e através de outras vias de sinalização acaba agindo como um regulador positivo do ciclo. O presente estudo visou comparar as expressões imunoistoquímicas das proteínas p27 e c-jun, através do método da estreptavidina-biotina em 45 casos de displasia epitelial agrupados em diferentes graus (atipia discreta, moderada e intensa), e 20 casos de carcinoma epidermóide intra-oral, na tentativa de elucidar o papel e a relação existente entre estas proteínas. Foi observado uma diminuição dos níveis de p27 e um aumento significativo da c-jun nos casos com graus elevados de displasia epitelial e carcinoma epidermóide. A análise estatística indicou uma correlação inversa estatísticamente significativa entre as expressões de p27 e c-jun. Os resultados sugerem as alterações destas proteínas como um importante evento no processo de carcinogênese intra-oral.
Title in English
Immunohistochemical comparison between the p27 and c-jun proteins expression in the oral carcinogenesis
Keywords in English
Carcinoma epidermóide
Displasia epitelial
Imunoistoquímica
p27 c-jun
Abstract in English
The expression of many cell cycle proteins has been studied in a number of malignant lesions. Among the main ones is the p27, which is encoded by the same name tumor suppressor gene, and it is altered in carcinomas, including oral cancers. Another protein that plays a role in the cell cycle control is the c-jun, which is encoded by a proto-oncogene, and acts as a positive regulator of the cell cycle, through other signaling pathways. The present study aimed to compare the immunohistochemical expression of p27 and c-jun proteins, using the streptavidinbiotin method, in 45 cases of epithelial dysplasia, classified in different degrees (mild, moderate and intense), and 20 cases of oral squamous cells carcinoma, in an attempt to elucidate the role and the relationship between these proteins. It was observed a decrease of p27 levels and a substantial increase of c-jun in cases with the highest degrees of epithelial dysplasia. The statistical analysis showed a significant inverse correlation between p27 and c-jun expressions. These results suggest these proteins alterations as an important event in the carcinogenic process. p27, c-jun, Immunohistochemistry, Squamous cells carcinoma, Epithelial dysplasia
 
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Publishing Date
2010-01-28
 
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