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Doctoral Thesis
DOI
10.11606/T.23.2011.tde-06032012-161940
Document
Author
Full name
Roberto Anaximandro Garcia Rejas
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2011
Supervisor
Committee
Sousa, Suzana Cantanhede Orsini Machado de (President)
Cury, Sérgio Elias Vieira
Marques, Yonara Maria Freire Soares
Nunes, Fabio Daumas
Pinto Júnior, Décio dos Santos
Title in Portuguese
Expressão das proteinas c-jun, junb, JNK e pc-jun no carcinoma adenóide cístico e carcinoma epidermóide da cavidade bucal
Keywords in Portuguese
Carcinoma adenóide cístico
Carcinoma de células escamosas
Proteínas proto-oncogênicas
Abstract in Portuguese
O carcinoma adenóide cístico e o carcinoma epidermóide são neoplasmas de origem epitelial que afetam a cavidade oral. O carcinoma adenóide cístico pode apresentar-se em glândulas salivares maiores e menores, possue alta propensão de invasão perineural e o padrão de infiltracão: sólido, tubular e cribriforme. O carcinoma epidermóide foi descrito como um processo multifatorial envolvendo agentes físicos, químicos e virais, capazes de afetar o metabolismo celular e induzir a proliferação neoplásica. As proteínas c-jun e junB são membros da familia JUN, capazes de homodimerizar ou heterodimerizar com c-fos ou com outras proteinas bzip. Evidências das funções específicas das subunidades do AP-1 foram mostradas por c-jun e junB, que podem atuar antagonicamente ou não no controle da transformação celular, diferenciação e expressão do AP-1 dependente do gene alvo. Mas a função de ambos é complexa e pode depender do tipo celular. A cJun N Terminal quinase (JNK) é um importante regulador positivo e/ou negativo do AP1. O objetivo deste estudo foi avaliar a expressão das proteínas c-jun, pcjun, junB e JNK em carcinoma adenóide cístico de glândula salivar e no carcinoma epidermóide da cavidade oral, através das técnicas de imunohistoquímica, imunofluorescência e western-blotting, em biopsias de tecido e linhagens celulares provenientes destas lesões. Os resultados evidenciaram a expressão da proteina cjun no carcinoma adenoide cistico e de cjun e junB no carcinoma epidermóide. Nao foi detectada a expressao de JNK nas neoplasias estudadas. A expressão destas proteínas no carcinoma adenóide cístico e no carcinoma epidermoide sugere que estas participam na progressão tumoral e/ou tumorigênese destas neoplasias compartilhando uma via em comum.
Title in English
c-jun, junB, JNK and pc-jun protein expression in adenoid cystic carcinoma and squamous cell carcinoma of the oral cavity
Keywords in English
Adenoid cystic carcinoma
Protooncogene
Squamous cell carcinoma
Abstract in English
Adenoid cystic carcinoma and squamous cell carcinoma are epithelial neoplasms that occur in the oral cavity. Adenoid cystic carcinoma can appear in minor and major salivary glands and it presents a high propensity to invade perineural areas and can show different patterns of growth: solid, tubular and cribiform. Squamous cell carcinoma was described as a multifactorial process involving chemical, physical and viral agents which are able to affect the celular metabolism and induce neoplasic proliferation The proteins cjun and junB are members of the JUN family able to homodimerizes or heterodimerizes with cfos and other bzip proteins. Evidence of specific functions of AP1 subunits was shown for cjun and junB that can act anthagonically or not on the control of celular transformation, differentiation and expression of AP1 depending on the target gene. But the way both of them function is complex and may depend on the cellular type. cJun N terminal quianse (JNK) is an important positive or negative regulator of AP1 The aim of this study is to evaluate the expressions of cjun, pcjun junB and JNK in the adenoid cystic carcinoma of salivary glands and squamous cell carcinoma from the oral cavity through inmunohistotochemistry, inmunofluorescence and western blot techniques in tissue biopsy and cell lines from both tumors. The results will show the expression of cjun protein in the adenoid cyctic carcinoma and cjun and junB expression in the squamous cell carcinoma. JNK expression was not detected in the studied tumors. The expression of these proteins in adenoid cystic and squamous cell carcinoma suggests that they participate on the tumor progression and tumorigenesis of these neoplasms, that can share a common pathway
 
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Publishing Date
2012-03-21
 
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