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Doctoral Thesis
DOI
https://doi.org/10.11606/T.23.2005.tde-13062005-115943
Document
Author
Full name
Walter Luiz Siqueira Junior
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2004
Supervisor
Committee
Nicolau, Jose (President)
Gomes, Ana Maria Martins
Mustacchi, Zan
Santos, Maria Teresa Botti Rodrigues dos
Santos, Marinilce Fagundes dos
Title in Portuguese
"Estudo de alguns parâmetros salivares em indivíduos com síndrome de DOWN"
Keywords in Portuguese
Ácido siálico
Amilase
Cálcio
Capacidade tampão
Fluxo salivar
Fósforo
Glândula salivar
Magnésio
Peroxidase
pH
Potássio
Proteína total
Saliva
Síndrome de Down
Sódio
Zinco
Abstract in Portuguese
O objetivo deste estudo foi mensurar o fluxo salivar, pH, capacidade tampão, concentrações de proteína total e ácido siálico, atividades das enzimas amilase e peroxidase e concentração dos íons sódio, potássio, cálcio, fósforo, zinco e magnésio em saliva total de indivíduos síndrome de Down com idade entre 1 e 25 anos. Nos indivíduos com idade entre 1 e 5 anos a saliva total foi coletada através de uma leve sucção, enquanto que nos outros indivíduos com idade entre 6 e 10, 11 e 15, 15 e 20, 21 e 25 a saliva total foi coletada com estimulação mecânica através da mastigação de parafilm, durante 10 minutos. O pH e a capacidade tampão foramdeterminadas usando um pHmetro digital. A capacidade tampão foi mensurada através de titulação com HCl a 0,01N. A concentração de eletrólitos foi determinada através de um espectrofotômetro de emissão atômica com fonte de excitação de argônio induzido. A proteína total foi mensurada através do reagente de Folin. A atividade da amilase foi mensurada através da produção de maltose e a atividade da peroxidase foi mensurada através da utilização de orto-dianisidina. Para a analise estatística os dados foram apresentados em media ± desvio padrão. Foi utilizado o teste “t" de Student para determinar as diferenças entre as medias dos indivíduos síndrome de Down e o grupo controle. Nenhuma diferença significante foi observada na concentração de ácido siálico, fósforo, zinco, magnésio e cálcio entre os indivíduos síndrome de Down e o grupo controle. A concentração de sódio, proteína total e a capacidade tampão demonstraram ser maior nos indivíduos com síndrome de Down em comparação ao grupo controle. Por outro lado, o fluxo salivar, a concentração de potássio, e a atividade das enzimas amilase e peroxidase foram menores no grupo síndrome de Down quando comparado ao grupo controle. Estes resultados sugerem que as pessoas com síndrome de Down apresentam alterações no metabolismo do ducto e/ou das células acinares das glândulas salivares.
Title in English
Study measurement the flow rate concenntration whole saliva of individuals with Down syndrome.
Keywords in English
Amylase
Buffer capacity
Calcium
Down syndrome
Flow rate
Magnesium
Peroxidase
pH
Phosphorus
Potassium
Saliva
Salivary gland
Sialic acid
Sodium
Total protein
Zinc
Abstract in English
The aim of this study was to measure the flow rate, pH, buffer capacity, sialic acid, total protein concentrations, amylase and peroxidase activities and sodium, potassium, calcium, phosphorus, zinc and magnesium concentration whole saliva of individuals with Down syndrome aged 1 - 25 years. In individuals aged 1-5 years the whole saliva was collected under slight suction, while in the others individuals aged 6-10, 11-15, 15-20, 21-25 the whole saliva was collected with stimulation by chewing a piece of parafilm, for 10 minutes. The pH and the buffer capacity were determined using a digital pHmeter. The buffer capacity was measured by titration with 0.01 N HCl. Electrolyte concentrations were determined by inductively coupled argon plasma with atomic emission spectrometry. Sialic acid was determined by thiobarbituric acid assay. Protein was determined by the folin’s phenol reagent. Amylase was assayed measuring the maltose produced by the breakdown of starch and peroxidase with ortho dianisidine. For statistical analysis the date are presented as mean ± SD. Student’s “t" test was used to determine differences between the mean of the Down syndrome and control groups. No statistically significant differences were observed in sialic acid, phosphorus, zinc, magnesium and calcium concentration between the individuals with Down syndrome and control group. The sodium and total protein concentration and buffer capacity showed higher in the Down syndrome than in the control group. On the other hand the flow rate and potassium concentration, amylase and peroxidase activities were lower in the Down syndrome than in the control group. These results suggest that the Down syndrome persons present alteration in the metabolism of the duct and/or acinar cells of salivary glands.
 
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Publishing Date
2005-10-14
 
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