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Master's Dissertation
DOI
10.11606/D.18.2007.tde-05062007-162213
Document
Author
Full name
Érika Lamaro Sarti
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Carlos, 2007
Supervisor
Committee
Silva, Maria Bernadete Amancio Varesche (President)
Cammarota, Magali Christe
Duarte, Iolanda Cristina Silveira
Title in Portuguese
Influência do oxigênio no crescimento de arquéias metanogênicas e bactérias redutoras de sulfato em reatores anaeróbios em batelada
Keywords in Portuguese
Arquéias metanogênicas
Bactérias redutoras de sulfato
FISH
Oxigênio
Reatores em batelada
Abstract in Portuguese
Este trabalho teve como objetivo avaliar o comportamento de arquéias metanogênicas e bactérias redutoras de sulfato na presença de oxigênio, em reatores anaeróbios em batelada, sob condições sulfetogênicas e mesofílicas. Os reatores foram inoculados com lodo anaeróbio proveniente de reator UASB utilizado no tratamento de água residuária de avicultura. Triplicatas de reatores foram alimentadas com meio basal ZINDER, acrescido de acetato de sódio, etanol ou lactato de sódio e sulfato de sódio, de modo a se obter relações DQO/sulfato iniciais de 1,1-1,5. O headspace dos reatores foi preenchido com 'N IND.2' (100%) acrescido de oxigênio puro comercial (3-3,5 mg/L de oxigênio dissolvido). Os reatores anaeróbios controle foram alimentados com os mesmos substratos orgânicos, entretanto, seu headspace foi preenchido com 'N IND.2'/'CO IND.2' (70/30%). Nos reatores com acetato de sódio, etanol e lactato de sódio as velocidades de produção de metano foram de 0,30 mmol/L.h, 0,41 mmol/L.h e 0,16 mmol/L.h, respectivamente, para os reatores controle. Em relação aos reatores com oxigênio os valores foram de 0,27 mmol/L.h, 0,40 mmol/L.h e 0,08 mmol/L.h, respectivamente. Na presença de acetato, etanol e lactato a redução de sulfato foi de 57% e 97%; 59,6% e 76,6%; 77,5% e 41,9%, respectivamente, nos reatores controle e com oxigênio. Nos reatores com acetato e etanol houve predomínio de organismos do domínio Archaea nos reatores controle (59,2% e 58,8%) e com oxigênio (60,7% e 54,5%), respectivamente. No reator controle contendo lactato, também ocorreu o predomínio de arquéias metanogênicas (56,2%), enquanto na presença de oxigênio houve predomínio de organismos do domínio Bacteria (63,9%). As proporções de BRS nos reatores controle e com oxigênio contendo acetato, etanol e lactato foram de 22% e 17,3%; 28% e 16,9%; 19,5% e 21,9%, respectivamente. O oxigênio não inibiu a metanogênese e nem a redução de sulfato nos reatores contendo acetato e etanol.
Title in English
Oxygen influence on methanogenic archaea and sulfate reducing bacteria's growth in anaerobic batch reactors
Keywords in English
Batch reactors and FISH
Methanogenic archaeas
Oxygen
Sulfate reducing bacteria
Abstract in English
This work aimed to evaluate the behavior of methanogenic archaea and sulfate reducing bacteria in the presence of oxygen, using anaerobic batch reactors under sulfidogenic and mesophilic conditions. The reactors were inoculated with anaerobic sludge from UASB reactor treating poultry wastes. Third copies of the reactors were fed with ZINDER medium, increased with sodium acetate, ethanol or sodium lactate and sodium sulfate, in order to get COD/sulfate ratio of 1,1-1,5. The headspace of the reactors was filled with 'N IND.2' (100%) and increased with oxygen (OD concentration of 3-3.5 mg/L). The anaerobic control reactors were fed with the same organics substrates, however, the headspace was filled with 'N IND.2'/'CO IND.2' (70/30%). The rates of methane production were 0.30 mmol/L.h, 0.41 mmol/L.h and 0.16 mmol/L.h, in acetate, ethanol and lactate controls reactors, respectively. In oxygen reactors, the rates of methane production were 0.27 mmol/L.h, 0.40 mmol/L.h and 0.08 mmol/L.h in acetate, ethanol and lactate reactors, respectively. The rates of sulfate reduction in acetate, ethanol and lactate reactors were 57% and 97%; 59.6% and 76.6%; 77.5% and 41.9%, respectively, in control reactors and oxygen reactors. In acetate and ethanol reactors, were verified predominance of Archaea domain in control reactors (59.2% and 58.8%) and oxygen reactors (60.7% and 54.5%), respectively. In lactate control reactor also was verified predominance of Archaea domain (56.2%), whereas in lactate oxygen reactors there was predominance of cells belonging to Bacteria domain (63.9%). BRS ratio in acetate, ethanol and lactate control reactors and oxygen reactors corresponded to 22% and 17.3%; 28% and 16.9%; 19.5% and 21.9%, respectively. The addition of oxygen didn't inhibit the methanogenesis and sulfate reduction in acetate reactors and in ethanol reactors.
 
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Dissertacao.pdf (1.50 Mbytes)
Publishing Date
2007-06-20
 
WARNING: The material described below relates to works resulting from this thesis or dissertation. The contents of these works are the author's responsibility.
  • HIRASAWA, J. S., et al. Application of molecular techniques to evaluate the methanogenic archaea and anaerobic bacteria in the presence of oxygen with diffrent COD:sulfate ratios in a UASB reactor. Anaerobe (London), 2008, vol. 14, p. 209-218.
  • HIRASAWA, J. S., et al. Aplicação de técnicas de biologia molecular para avaliar a influência do oxigênio no crescimento de bactérias redutoras de sulfato e arquéias metanogênicas no reator UASB. In Sinaferm 2007 - XVI Simpósio Nacional de Bioprocessos, Curitiba - Paraná, 2007. Sinaferm 2007 - XVI Simpósio Nacional de Bioprocessos., 2007.
All rights of the thesis/dissertation are from the authors
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