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Doctoral Thesis
DOI
https://doi.org/10.11606/T.17.1983.tde-08032024-111214
Document
Author
Full name
Augusto Adam Netto
Institute/School/College
Knowledge Area
Date of Defense
Published
Ribeirão Preto, 1983
Supervisor
Committee
Romao, Erasmo (President)
Bicas, Harley Edison Amaral
Coutinho Netto, Joaquim
Ferreira, Affonso Luiz
Freusberg, Otto
Title in Portuguese
Efeitos da ação prolongada da luz fluorescente sobre o bulbo ocular, anexos oculares e glândulas pineal e supra-renal de ratos albinos recém-nascidos
Keywords in Portuguese
Bulbo ocular
Glândulas pineal e supra-renal
Luz fluorescente
Ratos albinos
Abstract in Portuguese
No presente trabalho estudou-se o efeito crônico da luz fluorescente contínua sobre os pesos corporal, dos bulbos oculares e das glândulas supra-renais; a histologia da retina, músculos extraoculares, glândulas de Harder, nervos ópticos, glândulas pineais e glândulas supra-renais; a espessura da retina; o número de células ganglionares da retina e o volume nuclear das células parenquimatosas da glândula pineal e do córtex da glândula supra-renal, de ratos albinos recém-nascidos. Para tal, 37 ratos albinos recém-nascidos, de ambos os sexos, com peso aproximado de 5,0g, divididos em 5 sub-grupos, foram mantidos numa caixa transparente de "plexiglass" e submetidos à iluminação contínua de 6 lâmpadas fluorescentes, "luz do dia", 20 watts, sob temperatura média de 30,5ºC, durante 5, 10, 17, 21 e 100 dias. Igual número de animais, de ambos os sexos e mesmo peso e idade, divididos igualmente em 5 sub-grupos, mantidos em iluminação natural solar cíclica e temperatura ambiental e sacrificados com 5, 10, 17, 21 e 100 dias de idade, constituíram o grupo controle. Até a ocasião do desmame a mãe foi mantida junto dos filhotes e água e alimentação foram fornecidas "ad libitum" aos dois grupos de animais. Constatou-se haver nos animais iluminados: diminuição dos pesos corporal, dos bulbos oculares e das glândulas supra-renais; atrofia da retina e redução da espessura das camadas retinianas; redução da espessura, degeneração e infiltração linfocitária dos músculos extraoculares; degeneração e infiltração linfocitária das glândulas de Harder; redução da espessura, edema das fibras nervosas e degeneração dos núcleos das células da oligodendroglia dos nervos ópticos; atrofia das glândulas pineais, com aparente redução do volume citoplasmático e degeneração dos núcleos das células parenquimatosas; atrofia das 3 zonas do córtex das glândulas supra-renais, com aparente redução do volume citoplasmático e degeneração dos núcleos celulares; redução da espessura central e periférica da retina; diminuição do número de células ganglionares e diminuição do volume nuclear das células parenquimatosas da glândula pineal e das células das 3 zonas do córtex da glândula supra-renal. Foram discutidos os possíveis mecanismos etiológicos responsáveis pelas alterações encontradas, em confronto com os achados existentes na literatura.
Title in English
Effects of prolonged action of fluorescent light on the ocular bulb, ocular annexes and pineal and adrenal glands of newborn albino rats
Keywords in English
Albino rats
Eye bulb
Fluorescent light
Pineal and adrenal glands
Abstract in English
The author searched the effects of continuous fluorescent light on weight of the body, eyeball and adrenal glands; histology of the retina, extraocular muscles, Harderian glands, optic nerves, pineal glands and adrenal glands; thickness of the retina; ganglion cells number in the retina and the nuclear volume of parenchimatous cells of pineal glands and adrenal cortex cells of newborn albino rats. With these purposes, thirty-seven newborn albino rats, female and male, weighting about 5.0g, divided into five sub-groups, were kept in a clear polyethylene cage, under illumination from six fluorescent lamps, daylight, 20 watts, during five, ten, seventeen, twenty-one and hundred days. The temperature in the cage was about 30.5ºC. Identical number of animals, female and male, having the same weight and age, also divided in five sub-groups, kept under cyclic solar illumination and enviromental temperature, were sacrified within five, ten, seventeen, twenty-one and hundred days, as the control group. After weanling, food and water was given ''ad libitum" to the animals. The animals exposed to the fluorescent light showed redution of body, eyeball and adrenal glands weight; retina atrophy and reduction in thickness of retinal layers; thickness reduction, degeneration and lymphocytic infiltration of extraocular muscles; degeneration and lymphocytic infiltration of Harderian glands; thickness reductjon, oedema of nerve fibers and degeneration of oligodendroglia cells in optic nerves; atrophy of pineal glands with an apparent reduction of citoplasmatic volume and nuclear degeneration of parenchimatous cells; atrophy of the three zones from the adrenal cortex with an apparent reduction of citoplasmatic volume and degeneration of cell's nucleous; central and peripheral retinal thickness reduction; reduction in number of retinal ganglion cells and reduction of nuclear volume of pineal parenchimatous cells and from all cells of the three zones of adrenal cortex. Finally, the author discussed the possibles mechanisms related to these alterations and compared his findings with those described in the correlated literature.
 
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