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Master's Dissertation
DOI
https://doi.org/10.11606/D.17.2020.tde-04012021-120026
Document
Author
Full name
Ana Beatriz dos Anjos Souza
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
Ribeirão Preto, 2020
Supervisor
Committee
Figueiredo, Luiz Tadeu Moraes (President)
Levi, José Eduardo
Quintana, Victor Hugo Aquino
Title in Portuguese
Estudo sobre infectividade dos vírus Chikungunya e Mayaro em hemocomponentes
Keywords in Portuguese
Alphavirus Chikungunya
Alphavirus Mayaro
Arboravírus
Hemocomponentes
Viremia
Abstract in Portuguese
Os arbovírus são responsáveis por doenças, esporádicas ou em epidemias, que podem ser graves ou debilitantes, com impacto significativo para a saúde humana no Brasil e no mundo, tornando importante o estudo de suas transmissibilidades por transfusão sanguínea. Dentre os arbovírus, os Alphavirus Chikungunya e Mayaro, apesar de não serem altamente letais, após produzirem doenças febris agudas, podem ser causadores de doenças articulares dolorosas e incapacitantes que perduraram por meses ou anos. No presente estudo, objetivou-se avaliar a infectividade dos Alphavirus Chikungunya e Mayaro em produtos do sangue nas unidades formadoras de plaques (PFU/mL) e por qPCR em número de cópias de RNA viral equivalente a PFU. Também pesquisou-se a infecção por Alphavirus Chikungunya e Mayaro em leucócitos, como linfócitos B, linfócitos T, monócitos e macrófagos do sangue periférico. Ainda, avaliou-se a infectividade dos dois vírus em linhagens de linfócitos T CD4+ (A30-1), de monócitos (THP-1) e de megacariócitos (MEG-1). Os resultados obtidos mostram que bolsas de sangue doado inoculadas com vírus Mayaro ou vírus Chikungunya, mantêm ambos os vírus infectantes durante o processamento dos hemoderivados (concentrado de hemácias, concentrado de plaquetas e plasma fresco congelado) e mesmo após o armazenamento específico recomendado para cada produto, sendo capazes de infectar indivíduos com eles transfundidos. Observou-se que o Mayaro e o Chikungunya infectam linfócitos B (CD19+ CD20+ ), linfócitos T (CD3+ e CD4+ ) e ainda, o Mayaro infecta monócitos (CD14+ ) e o Chikungunya infecta macrófagos (CD68+ ) do sangue periférico, podendo ser disseminados nos pacientes por estas células. Também o vírus Mayaro e o Chikungunya infectam culturas de linfócitos TCD4+ , megacariócitos e monócitos humanos, produzindo relevantes replicações virais que têm possíveis repercussões na fisiopatologia das doenças por estes vírus. Os resultados deste trabalho evidenciam que, para prevenir infecções por vírus Mayaro e por vírus Chikungunya em hemoderivados, faz-se necessário estimular o desenvolvimento e a utilização de testes diagnósticos para estes patógenos no sangue doado.
Title in English
Study on the infectivity of Chikungunya and Mayaro virus in blood components
Keywords in English
Alphavirus Chikungunya
Alphavirus Mayaro
Arboravirus
Hemocomponents
Viremia
Abstract in English
Arboviruses are responsible for diseases, sporadic or in epidemics, which can be serious or debilitating with a significant impact on human health in Brazil and worldwide making it important to study their transmissibilities through blood transfusion. Among the arboviruses, the Alphaviruses Chikungunya and Mayaro, although not highly lethal, after producing acute febrile illnesses can cause painful and disabling joint diseases that lasted for months or years. In the present study the objective was to evaluate the infectivity of Alphavirus Chikungunya and Mayaro in blood products in plaque forming units (PFU / mL) and by qPCR in number of copies of PFU-equivalent viral RNA. Alphavirus Chikungunya and Mayaro infection was also investigated in leukocytes, such as B lymphocytes, T lymphocytes, monocytes and macrophages of peripheral blood. In addition, the infectivity of the two viruses in CD4 + T lymphocyte (A30-1), monocyte (THP-1) and megakaryocyte (MEG-1) lines was evaluated. The results obtained show that donated blood bags inoculated with Mayaro virus or Chikungunya virus keep both infecting viruses during the processing of blood products (red blood cell concentrate, platelet concentrate and fresh frozen plasma) and even after the specific storage recommended for each product, being able to infect individuals transfused with them. It was observed that Mayaro and Chikungunya infect B lymphocytes (CD19 + CD20 +), T lymphocytes (CD3 + and CD4 +) and Mayaro infects monocytes (CD14 +) and Chikungunya infects peripheral blood macrophages (CD68 +), which can be disseminated in patients by those cells. Mayaro virus and Chikungunya also infect cultures of TCD4 + lymphocytes, megakaryocytes and human monocytes, producing relevant viral replications that have possible repercussions on the pathophysiology of diseases caused by these viruses. The results of this work show that to prevent infections by Mayaro virus and Chikungunya virus in blood products, it is necessary to stimulate the development and use of diagnostic tests for these pathogens in donated blood.
 
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Publishing Date
2021-01-29
 
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