• JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
 
  Bookmark and Share
 
 
Doctoral Thesis
DOI
https://doi.org/10.11606/T.17.2021.tde-28012022-112417
Document
Author
Full name
Stephanya Covas da Silva
Institute/School/College
Knowledge Area
Date of Defense
Published
Ribeirão Preto, 2021
Supervisor
Committee
Lopes, Luiza da Silva (President)
Fazan, Valeria Paula Sassoli
Feres, Omar
Hamamoto Filho, Pedro Tadao
Title in Portuguese
Análise dos efeitos da oxigenoterapia hiperbárica associada ao tratamento cirúrgico em lesões cerebrais secundárias à hidrocefalia experimental
Keywords in Portuguese
Derivação liquórica
Hidrocefalia
Isquemia encefálica
Oxigenoterapia hiperbárica
Abstract in Portuguese
A hidrocefalia é caracterizada pelo acúmulo de líquor dentro dos ventrículos cerebrais e do espaço subaracnoideo. O volume ventricular pode aumentar progressivamente e gerar graves lesões ao sistema nervoso, tendo a hipóxia/isquemia cerebral como um dos mais importantes fatores envolvidos. A oxigenoterapia hiperbárica (OHB) tem como principal efeito a melhora do aporte de oxigênio aos tecidos, o que pode diminuir a progressão das lesões secundárias ao aumento ventricular. O objetivo deste trabalho foi avaliar se a OHB associada à cirurgia de derivação liquórica promove efeitos neuroprotetores para as estruturas lesadas de forma secundária à hidrocefalia e compreender sua atuação. Foram utilizados ratos machos da linhagem Wistar Hannover com sete dias de vida submetidos à hidrocefalia por injeção intracisternal de caulim 15%. Contendo dez animais em cada, seis grupos foram formados: controle, controle associado à terapia hiperbárica, hidrocefálico sem tratamento, hidrocefálico tratado com oxigenoterapia hiperbárica (3 ATA/2h/dia), hidrocefálico tratado com derivação liquórica e hidrocefálico tratado com oxigenoterapia hiperbárica (3 ATA/2h/dia) associada à derivação liquórica. Para avaliação da resposta ao tratamento foram realizados testes de comportamento (campo aberto, labirinto aquático de Morris modificado e reconhecimento de objetos), avaliação por ultrassonografia transcraniana, estudos histológicos (hematoxilinaeosina e luxol fast blue), imunoistoquímicos (GFAP, Ki-67, Caspase-3, COX-2, NeuN e SOD1) e bioquímicos para quantificação das proteínas GFAP e MBP através do método ELISA. Os resultados mostram que a associação dos tratamentos exerce efeitos neuroprotetores como a melhora neurocomportamental, preservação das estruturas periventriculares e redução de danos decorrentes da isquemia e do processo inflamatório. Concluímos que a oxigenoterapia hiperbárica possui potencial para ser utilizada como tratamento associado à cirurgia de derivação liquórica na hidrocefalia experimental.
Title in English
Analysis of the effects of hyperbaric oxygen therapy associated with surgical treatment in brain injuries secondary to experimental hydrocephalus
Keywords in English
Brain ischemia
CSF shunt
Hydrocephalus
Hyperbaric oxygen therapy
Abstract in English
Hydrocephalus is characterized by the accumulation of CSF inside the cerebral ventricles and the subarachnoid space. Ventricular volume can progressively increase and generate serious damage to the nervous system, with cerebral hypoxia/ischemia as one of the most important factors involved. Hyperbaric oxygen therapy (HBOT) has as its main effect the improvement of oxygen supply to tissues, which can reduce the progression of injuries secondary to ventricular enlargement. This study aimed to evaluate whether HBOT associated with CSF shunt surgery promotes neuroprotective effects for injured structures secondary to hydrocephalus and to understand its role. Seven-day-old male Wistar Hannover rats submitted to hydrocephalus by intracisternal injection of 15% kaolin were used. Containing ten animals in each, six groups were formed: control, control associated with hyperbaric therapy, hydrocephalic without treatment, hydrocephalic treated with hyperbaric oxygen therapy (3 ATA/2h/day), hydrocephalic treated with CSF derivation, and hydrocephalic treated with hyperbaric oxygen therapy (3 ATA/2h/day) associated with CSF derivation. To assess the response to treatment, behavioral tests were performed (open field, modified Morris water maze and object recognition), evaluation by transcranial ultrasonography, histological studies (hematoxylin-eosin and Luxol fast blue), immunohistochemical (GFAP, Ki-67, Caspase-3, COX-2, NeuN, and SOD1) and biochemicals for quantification of GFAP and MBP proteins through the ELISA method. The results show that the combination of treatments exerts neuroprotective effects such as neurobehavioral improvement, preservation of periventricular structures, and reduction of damage resulting from ischemia and the inflammatory process. We conclude that hyperbaric oxygen therapy has the potential to be used as a treatment associated with CSF shunt surgery in experimental hydrocephalus.
 
WARNING - Viewing this document is conditioned on your acceptance of the following terms of use:
This document is only for private use for research and teaching activities. Reproduction for commercial use is forbidden. This rights cover the whole data about this document as well as its contents. Any uses or copies of this document in whole or in part must include the author's name.
Publishing Date
2022-02-18
 
WARNING: Learn what derived works are clicking here.
All rights of the thesis/dissertation are from the authors
CeTI-SC/STI
Digital Library of Theses and Dissertations of USP. Copyright © 2001-2022. All rights reserved.