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Master's Dissertation
DOI
10.11606/D.17.2019.tde-19022019-113140
Document
Author
Full name
Mucio Luiz de Assis Cirino
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
Ribeirão Preto, 2018
Supervisor
Committee
Tirapelli, Daniela Pretti da Cunha (President)
Schimming, Bruno Cesar
Lizarte Neto, Fermino Sanches
Peria, Fernanda Maris
Title in Portuguese
Expressão dos microRNAs miR-15, miR-29, miR219 e miR-222 em ratos submetidos a isquemia cerebral focal associada ao exercício físico
Keywords in Portuguese
Exercício físico
Isquemia cerebral
microRNAs
Neurotransmissores
Abstract in Portuguese
INTRODUÇÃO: A isquemia cerebral é uma das principais causas de morte no Brasil, segundo levantamento da Sociedade Brasileira de Neurologia em 2000, sendo a terceira causa de morte após doenças cardiovasculares e o câncer, além de ser uma das maiores causas de sequela permanente capaz de gerar incapacidade. Nas últimas décadas, estudos experimentais tem demonstrado efeitos benéficos do exercício físico associado à isquemia cerebral. Vários mecanismos moleculares estão envolvidos na fisiopatologia da isquemia cerebral, entre eles as alterações nos perfis de expressão de neurotransmissores. Pesquisas atuais também destacam o papel dos microRNAs na isquemia cerebral quanto na regulação dos neurotransmissores. Portanto, analisar a expressão de neurotransmissores e microRNAs associados à isquemia cerebral, assim como o papel dos benefícios promovidos pelo exercício físico poderá contribuir na elucidação de possíveis vias moleculares com efeito neuroprotetor. MATERIAIS E MÉTODOS: Foram utilizados 48 animais divididos em quatro grupos experimentais: controle, submetido à isquemia cerebral, submetido ao exercício físico e submetido ao exercício físico associado à isquemia cerebral. A metodologia de PCR em tempo real foi utilizada para analisar a expressão dos miRNAs; miR15b, miR29b, miR-219 e miR-222. RESULTADOS E CONCLUSÃO: não observamos diferenças estatísticas significativas na expressão dos miRNAs miR-15b, miR- 12 29b, miR-219 e miR-222 no tecido cerebral dos grupos submetidos à isquemia cerebral, submetidos ao exercício físico e na associação dos dois grupos quando comparados ao grupo controle. Entretanto, os miRNAs miR-15b e miR222 apresentaram maior expressão no grupo com a associação da isquemia cerebral e exercício físico
Title in English
Expression of miR-15, miR-29, miR-219 and miR222 microRNAs in rats submitted to physical exercise associated to focal cerebral ischemia
Keywords in English
Brain ischemia
microRNAs
Neurotransmitter
Physical exercise
Abstract in English
INTRODUCTION: Cerebral ischemia is one of the main causes of death in Brazil, according to a survey by the Brazilian Society of Neurology in 2000, being the third cause of death after cardiovascular diseases and cancer, besides being one of the major causes of permanent sequela capable of generate disability. In the last decades, experimental studies have shown beneficial effects of physical exercise associated with cerebral ischemia. Several molecular mechanisms are involved in the pathophysiology of cerebral ischemia, including changes in neurotransmitter expression profiles. Current research also highlights the role of microRNAs both in the process of cerebral ischemia and in the regulation of neurotransmitters. Therefore, analyzing the expression of neurotransmitters and microRNAs associated with cerebral ischemia, as well as the role of the benefits promoted by physical exercise may contribute to the elucidation of possible molecular pathways with neuroprotective effect. MATERIALS AND METHODS: 48 animals were divided into 4 experimental groups: control, cerebral ischemia, physical exercise and physical exercise associated with cerebral ischemia. The real-time PCR methodology was used to analyze miRNA expression: miR15b, miR-29b, miR219 and miR-222. RESULTS AND CONCLUSION: We did not observe statistically significant differences in the miRNA expression of miRNAs: miR 15b, miR-29b, miR-219 and miR-222 in brain tissue groups submitted to 15 cerebral ischemia, physical exercise and in the association of the two groups when compared to the group control. However, the miR-15b and miR-222 levels of expression increased in the group of cerebral ischemia associated with physical exercise
 
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Publishing Date
2019-04-02
 
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