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Doctoral Thesis
DOI
https://doi.org/10.11606/T.17.2005.tde-31082023-110235
Document
Author
Full name
Lenicy Lucas de Miranda Cerqueira
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
Ribeirão Preto, 2005
Supervisor
Committee
Contel, Eucleia Primo Betioli (President)
Freitas, Maria Armênia Ramalho de
Grisolia, Maria de Nazaré Klautau Guimarães
Lui, Jeffrey Frederico
Tonhati, Humberto
Title in Portuguese
Caracterização genética de javalis (Sus scrofa scrofa L.) através de marcadores microssatélites - STRs
Keywords in Portuguese
Iniciadores heterólogos
Javali
Marcadores microssatélites
Abstract in Portuguese
No início do século XX, foram trazidos alguns javalis para a Argentina, que acabaram escapando e se espalhando pelo norte da Argentina, Uruguai e Sul do Brasil. Na década de 90 começaram a ser importados alguns animais puros, para fins de criação comercial, principalmente da França e Canadá. Ocorrem no Brasil também animais híbridos com 2n= 37 ou 38 cromossomos, descendentes do cruzamento do javali (2n= 36) com o porco doméstico (2n= 38). O objetivo deste trabalho foi a caracterização genética dos javalis considerados puros, originários da Europa, criados em cativeiros no país, através de marcadores microssatélites, com o intuito de contribuir com o monitoramento da variabilidade genética e sendo assim, auxiliar no manejo e gerenciamento de programas de melhoramento genético. Para estudar a pureza das linhagens de javalis, assim como estimar a variabilidade genética dentro de alguns grupos, o DNA genômico foi extraído e posteriormente amplificado pela técnica de PCR para análise de microssatélites (IGF1, SW444, ACTG2, LOX12A, CGA e TNFB), os quais foram desenvolvidos para a subespécie Sus scrofa doméstica (suíno doméstico). As condições de amplificação foram padronizadas para o javali (Sus scrofa scrofa). Todos os seis loci foram polimórficos, apresentando um total de 36 alelos nos dois grupos genéticos (indivíduos com 2n= 36 e 2n= 37 ou 38 cromossomos). A utilização de iniciadores heterólogos é importante, pois, elimina a necessidade do desenvolvimento de biblioteca genômica, tornando-se um método mais rápido e de menor custo.
Title in English
Genetic characterization of wild boar (Sus scrofa scrofa L.) through microsatellite markers - STRs
Keywords in English
Heterologous primers
Microsatellite markers
Wild boar
Abstract in English
ln the beginning of the XXth century some wild boar had been brought to Argentina and from there they had escaped and spred to northern Argentina, Uruguay and southern Brazil. During the 1990s some pure animals were imported for commercial purposes, from France and Canada. Nowadays hybrid animals occur in Brazil (with 2n = 37 or 38 chromosomes) descendants from cresses between the wild boar (2n = 36) and the domestic pig (2n = 38). The objective of the present work was to study the genetic composition of wild boars considered from pure lineages coming from Europe, and maintained in captivity in Brazil. Microsatellite markers were used with the purpose to contribute with the monitoring of the genetic variability and to help in management and genetic improvement programs. The genomic DNA from wild boar was extracted and later amplified by PCR technique to be analyzed for 1GF1, SW444, ACTG2, LOX12A, CGA and TNFB microsatellites described for Sus scrofa domestica, the domestic pig. Amplification conditions were standardized for the wild boar (Sus scrofa scrofa), and all the six loci were polymorphic, showing a total of 36 alleles. The possibility to utilize heterologous primers is a very important condition because it eliminates the necessity to develop a genomic library and thus becoming a faster and cheaper method.
 
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Publishing Date
2023-08-31
 
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