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Master's Dissertation
DOI
https://doi.org/10.11606/D.17.2022.tde-20062022-161952
Document
Author
Full name
Gabriel Ernesto Silva Trentin
Institute/School/College
Knowledge Area
Date of Defense
Published
Ribeirão Preto, 2022
Supervisor
Committee
Almeida, Fausto Bruno dos Reis (President)
Rossi, Nilce Maria Martinez
Vasconcelos, Ana Flora Dalberto
Title in Portuguese
Identificação de metabólitos secundários presentes em vesículas extracelulares de Candida albicans durante estresse oxidativo
Keywords in Portuguese
Candida albicans
Fator de virulência
Metabólitos secundários
Metabolômica
Vesículas extracelulares
Abstract in Portuguese
Candida albicans é um fungo comensal em humanos saudáveis, podendo causar infecção em alguns indivíduos por meio da secreção de diversos fatores de virulência. O sucesso do estabelecimento da infecção se deve a estratégias elaboradas para lidar com moléculas defensivas secretadas pelo hospedeiro, incluindo a resposta ao estresse oxidativo. A liberação de vesículas extracelulares (VEs) é considerada uma alternativa ao mecanismo de secreção de biomoléculas que favorece a interação do fungo com o ambiente do hospedeiro e está associada a propriedades imunomoduladoras. Os estudos sobre metabólitos secundários em VEs são escassos. Desta forma, nosso objetivo foi caracterizar metabólitos secundários presentes em VEs de C. albicans sob condição de estresse oxidativo em comparação com a cultura controle, que consistiu no crescimento de C. albicans em meio de Sabouraud. Em relação à identificação de metabólitos contidos nas VEs, realizamos análises de Cromatografia Líquida acoplada a Espectrometria de Massa (LC-MS), identificando seis moléculas. Essas moléculas podem estar relacionadas a diversos processos biológicos, como as vias metabólicas de glicerofosfolipídios e esfingolipídeos, que podem atuar em diferentes níveis, sintonizando a produção de compostos de acordo com as necessidades das células que acabam por favorecer uma infinidade de respostas adaptativas. Nossos resultados demonstram nova compreensão sobre o papel das VEs na biologia fúngica e na interação hospedeiro-patógeno.
Title in English
Identification of secondary metabolites present in extracellular vesicles of Candida albicans during oxidative stress
Keywords in English
Candida albicans
Extracellular vesicles
Metabolomics
Secondary metabolites
Virulence factor
Abstract in English
Candida albicans is a commensal fungus in healthy humans and may cause infection in individuals through the secretion of several virulence factors. The success of infection establishment is due to strategies elaborate to cope with defensive molecules secreted by the host, including the response toward oxidative stress. The release of extracellular vesicles (EVs) is considered an alternative to the biomolecules secretory mechanism that favors the fungus interaction with the host's environment and is also associated with immunomodulatory properties. The studies about secondary metabolites in EVs are scarce. Our work characterized the content of secondary metabolites in C. albicans EVs under oxidative stress condition in comparison to control culture, which consisted of C. albicans growth in Sabouraud media. Regarding the identification of EV containing metabolites, we performed Liquid Chromatography coupled to Mass Spectrometry (LC-MS) analyses, retrieving six molecules. These molecules may be related to several biological processes such as glycerophospholipid and sphingolipids pathways, which may act at different levels by tuning the production of compounds in accordance to cells requirements that ultimately favor a myriad of adaptive responses. Taken together, our results showed new insights into the understanding of EVs roles on fungal biology and host-pathogen interaction.
 
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Publishing Date
2022-06-23
 
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