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Master's Dissertation
DOI
10.11606/D.11.2005.tde-16112005-165649
Document
Author
Full name
Carlos David Vera Bravo
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
Piracicaba, 2005
Supervisor
Committee
Goncalves, Antonio Natal (President)
Almeida, Marcilio de
Moraes, Mario Luiz Teixeira de
Title in Portuguese
Controle genético e histogênese na regeneração de progênies de Eucalyptus grandis in vitro
Keywords in Portuguese
controle genético
eucalipto
herdabilidade
histologia vegetal
organogênese
regeneração “in vitro”
Abstract in Portuguese
Com o objetivo de conhecer meios, explantes, tecidos envolvidos e controle genético da regeneração in vitro a partir de plântulas de Eucalyptus grandis, dez progênies de polinização aberta da população base, origem Atherton localizada em Anhembi, estado de São Paulo foram utilizadas. Sementes de sete progênies foram germinadas in vitro. Após vinte dias de cultivo, cotilédones, segmentos proximais e distais dos hipocótilos foram inoculados em nove meios de cultura usando o meio basal de JADS em combinação com dois reguladores de crescimento (ANA e BAP). Após 14 semanas de cultivo, foram feitas avaliações da regeneração e histologia. Células do córtex estavam envolvidos na regeneração dos hipocótilos e as células subepidérmicas originaram calos e estes deram origem a brotos nos cotilédones. Todos os brotos formados tiveram conexão com o sistema vascular do tecido original. Foi verificado que o melhor tratamento, onde 41% dos hipocótilos distais regeneraram, na combinação de ANA e BAP 0,5 mg L-1. Posteriormente, os segmentos distais dos hipocótilos das dez progênies foram inoculados naquele meio, houve diferenças significativas de regeneração entre as progênies (P<0,0001) com extremos de regeneração de 11% a 60% e com uma média de 37%. A herdabilidade quanto ao caráter foi alta (h 2 m =0,94), indicando que houve um forte controle genético na regeneração in vitro dentro da população. Houve também alta variabilidade dentro da amostra estudada assim como um forte efeito materno sobre a regeneração.
Title in English
Genetic control and histological analysis of in vitro regeneration of Eucalyptus grandis progenies
Keywords in English
eucalyptus grandis
genetic control
heritability
histology
in vitro regeneration
organogenesis
Abstract in English
Aiming at better knowledge on tissue culture media, axplants, tissues involved and genetic control of in vitro regeneration of Eucalyptus grandis from seedlings, seeds from ten open pollinated progenies from the basic population, origin Atherton, located in Anhembi, São Paulo State (BR) were used. Initially, seeds from seven progenies were germinated in vitro. Twenty days after culture, cotyledons and, distal and proximal hypocotyls segments were inoculated in culture media using JADS basic medium with varius NAA + BAP combinations. After 14 weeks of culture, evaluations bud induction, regeneration, and histological analysis were done. Cortex cells were involved in hypocotyls direct regeneration and cotyledon epidermic cells gave rise to callus which in turn produced shoots. All shoots developed had connection to the original vascular system. It was verified that NAA + BAP at 0,5 mgL-1 concentration each was the best treatment, where 41% of the distal hypocotyls regenerated. Distal hypocotyl segments from of seedlings of ten progenies were inoculated showing significant differences regeneration among progenies. Regeneration varied from 11% to 60% with a mean of 37%; the heritability in relation to the character was ( 94 , 0 2 = m h ), indicating that there was a strong genetic control of the in vitro regeneration within the population. Also, there was a high variability within the samples studied as well as a strong maternal effect on the regeneration.
 
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CarlosBravo.pdf (1.65 Mbytes)
Publishing Date
2005-11-19
 
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