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Doctoral Thesis
DOI
https://doi.org/10.11606/T.11.2021.tde-14022022-143313
Document
Author
Full name
Pedro Alexander Velasquez Vasconez
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
Piracicaba, 2022
Supervisor
Committee
Silva Filho, Marcio de Castro (President)
Dias, Renata de Oliveira
Fruttero, Leonardo Luis
Vitorello, Claudia Barros Monteiro
Title in Portuguese
Adaptação de Helicoverpa armigera aos inibidores de peptidase de soja envolve uma alteração no padrão de expressão das serino peptidases e sugere um controle epigenético
Keywords in Portuguese
Helicoverpa armigera
Epigenética
Insetos polífagos
Metilação do DNA
Plasticidade fenotípica
Quimotripsinas
Tripsinas
Abstract in Portuguese
Após décadas das primeiras publicações sobre a plasticidade transcricional do sistema digestivo dos insetos polífagos, ainda não se conhece em profundidade como os genes são regulados e as vias de sinalização envolvidas neste mecanismo adaptativo. Inicialmente, foi mostrado que a adaptação de lagartas de H. armigera à alimentação com inibidores de peptidase de soja (IPs) se deve à alteração do perfil transcricional de genes codificadores de serino peptidases do sistema digestivo. A presença dos IPs na dieta causou mudanças na regulação de genes de um amplo repertório de enzimas proteolíticas, principalmente com o aumento de genes de tripsinas. A regulação diferencial de enzimas digestivas foi associada à adaptação da H. armigera ao efeito anti-nutricional dos IPs. Além disso, a análise de dados de expressão gênica em larga escala (RNA-seq) mostrou que aproximadamente 40% dos transcritos (cerca de 500 epialelos potenciais) induzidos pelos IPs foram transmitidos à geração seguinte, apesar das lagartas desenvolverem-se em dietas desprovidas de IPs. A fim de caracterizar um possível mecanismo epigenético responsável por este mecanismo adaptativo, foi realizado o metiloma do sistema digestivo das lagartas da primeira geração (alimentadas com e sem IPs) e das progênies de lagartas expostas aos IPs. Epialelos de metilação do DNA foram associados aos controles transcricional e traducional, sugerindo um possível envolvimento da alteração do padrão de metilação na adaptação de H. armigera aos IPs. Este trabalho abre novas perspectivas no entendimento do polifagismo e a plasticidade dos insetos na alimentação de um amplo número de plantas hospedeiras, influenciando o seu curso evolutivo.
Title in English
Adaptation of Helicoverpa armigera to soy peptidase inhibitors involves an alteration in the expression pattern of serine peptidases and suggests an epigenetic control
Keywords in English
Helicoverpa armigera
Chymotrypsins
DNA methylation
Epigenetics
Phenotypic plasticity
Polyphagous insects
Trypsins
Abstract in English
After decades of the first publications on the transcriptional plasticity of the digestive system of polyphagous insects, it is not yet known in depth how genes are regulated and the signaling pathways involved in this adaptive mechanism. Initially, it was shown that the adaptation of Helicoverpa armigera larvae to feeding with soybean peptidase inhibitors (SPI) is due to the alteration of the transcriptional profile of genes encoding serine peptidases in the digestive system. The SPI in the diet caused changes in gene regulation of a wide repertoire of proteolytic enzymes, mainly with the increase of trypsin genes. Differential regulation of digestive enzymes was associated with the adaptation of H. armigera to the anti-nutritional effect of PIs. In addition, large- scale gene expression data analysis (RNA-seq) showed that approximately 40% of the transcripts (about 500 potential epialleles) induced by PIs were passed on to the next generation, even though larvae thrived on deprived diets of IPs. In order to characterize a possible epigenetic mechanism responsible for this adaptive mechanism, the methylome of the digestive system of first-generation larvae (fed with and without SPI) and of the progenies of larvae exposed to SPI was evaluated. DNA methylation epialleles were associated with transcriptional and translational controls, suggesting a possible involvement of the alteration of the methylation pattern in the adaptation of H. armigera to SPI. This work opens new perspectives on the understanding of polyphagism and the plasticity of insects in feeding many host plants, influencing their evolutionary course.
 
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Publishing Date
2022-02-16
 
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