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Doctoral Thesis
DOI
https://doi.org/10.11606/T.11.2023.tde-12092023-165632
Document
Author
Full name
Laecio Fernandes Souza Sampaio
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
Piracicaba, 2023
Supervisor
Committee
Mourao Filho, Francisco de Assis Alves (President)
Colombo, Carlos Augusto
Veasey, Elizabeth Ann
Zucchi, Maria Imaculada
Title in Portuguese
Genética populacional de espécies frutíferas nativas de Eugenia spp. (Myrtaceae) visando a conservação e o melhoramento
Keywords in Portuguese
Diversidade genética
Genotipagem por sequenciamento
Marcadores SNP
Mata Atlântica
Abstract in Portuguese
Espécies como uvaia (Eugenia pyriformis Cambess), grumixama (Eugenia brasiliensis Lam.) e cereja-do-Rio Grande (Eugenia involucrata DC.) despertam interesse por produzirem frutos aromáticos, saborosos, nutritivos e apresentarem inúmeros compostos fenólicos e antioxidantes naturais. O conhecimento sobre a diversidade genética e a estrutura populacional dessas espécies é limitado, o que dificulta o estabelecimento de estratégias de manejo, conservação, melhoramento e exploração desses recursos genéticos. No presente estudo, analisou-se a diversidade genômica e a estrutura populacional de acessos de uvaia, grumixama e cereja-do-Rio Grande depositados em coleções de instituições de pesquisa, utilizados para arborização urbana e mantidos por pequenos agricultores de São Paulo e Minas Gerais, utilizando marcadores de polimorfismo de nucleotídeo único (SNPs) originados de genotipagem por sequenciamento (genotyping by sequencing - GBS). Foram identificados 2299 SNPs entre acessos de grumixama, 2872 SNPs para uvaia e 1471 SNPs para cereja-do-Rio Grande. Os marcadores identificados foram eficazes na caracterização da diversidade genômica e estrutura populacional das três espécies em estudo. Os níveis de diversidade genômica e endogamia foram compatíveis com a biologia de cada espécie. Foi observada grande diferenciação genética entre as coleções dentro de cada uma das espécies. A aplicação de abordagens genômicas populacionais podem ser valiosas para o estabelecimento de melhores práticas de manejo dessas culturas, promovendo a conservação e valorização dos recursos genéticos nativos brasileiros.
Title in English
Population genetics of native fruit species of Eugenia spp. (Myrtaceae) for conservation and improvement
Keywords in English
Atlantic rainforest
Genetic diversity
Genotyping by sequencing
SNP markers
Abstract in English
Species such as uvaia (Eugenia pyriformis Cambess), grumixama (Eugenia brasiliensis Lam.), and cereja-do-Rio Grande (Eugenia involucrata DC.) have aromatic, tasty, nutritious fruits and have numerous phenolic compounds and natural antioxidants. Knowledge about the genetic diversity and population structure of these species is limited, which makes it difficult to establish management, conservation, improvement and exploitation strategies for these genetic resources. In the present study, we analyzed the genomic diversity and population structure of uvaia, grumixama and cereja-do-Rio Grande accessions deposited in collections of research institutions, used for urban afforestation and maintained by small farmers in the state of São Paulo and Minas Gerais, using markers of single nucleotide polymorphism (SNP) originated from genotyping by sequencing (GBS). We identified 2299 SNPs for grumixama, 2872 SNPs for uvaia and 1471 SNPs for cereja-do-Rio Grande. The identified markers were effective in characterizing the genomic diversity and population structure of the three species under study. The levels of genomic diversity and inbreeding were compatible with the biology of each species. Great genetic differentiation was observed between the collections within each of the species. The application of population genomic approaches can be valuable for the establishment of better management practices for these cultures, promoting the conservation and valorization of Brazilian native genetic resources.
 
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Release Date
2025-07-03
Publishing Date
2023-09-14
 
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