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Master's Dissertation
DOI
https://doi.org/10.11606/D.10.2023.tde-22022024-112244
Document
Author
Full name
Lucas Alencar Fernandes Beserra
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2023
Supervisor
Committee
Gregory, Lilian (President)
Carvalho, Jeferson Silva
Gil, Maria Angeles Ramo
Title in Portuguese
Detecção de Coxiella burnetii em abortos de fêmeas bovinas no Brasil
Keywords in Portuguese
Abortamento
Coxielose
Doenças reprodutivas
Ruminantes
Zoonoses
Abstract in Portuguese
As doenças reprodutivas de origem infecciosa impactam negativamente a expansão, desempenho, sanidade e lucratividade dos rebanhos nacionais. A Coxiella burnetii é uma bactéria intracelular obrigatória, zoonótica e amplamente difundida na natureza. Esse agente infecta uma grande variedade de hospedeiros, incluindo os ruminantes. Na espécie bovina, a coxielose é responsável por distúrbios reprodutivos. Desta forma, o objetivo desse trabalho foi investigar a presença da Coxiella burnetii em abortos de fêmeas bovinas no Brasil. Nesse estudo, foram utilizadas 126 amostras constituídas de pool fetal, timo, linfonodo, sistema nervoso central e placentas. O diagnóstico foi realizado através da técnica de PCR quantitativo utilizando o kit comercial EXOone Coxiella burnetii®. Foram detectadas 20 amostras positivas das 126 analisadas. Das amostras que resultaram positivas 15 eram de pool fetal, 3 de timo e 2 de placentas. Neste estudo foi detectado pela primeira vez, a Coxiella burnetii em timo fetal, e no Brasil em placenta. Diante desses resultados, demonstra-se que há a circulação do patógeno no país e medidas de diagnóstico e controle da enfermidade devem ser elaboradas. Devido aos riscos à saúde pública associada a atividade ocupacional e exposição a Coxiella burnetii é necessário a implementação de medidas de vigilância em saúde única.
Title in English
Detection of Coxiella burnetii in abortions of bovine females in Brazil
Keywords in English
Abortion
Coxiellosis
Reproductive diseases
Ruminants
Zoonoses
Abstract in English
Reproductive diseases of infectious origin negatively impact the expansion, performance, health and profitability of national herds. Coxiella burnetii is an obligatory intracellular bacterium, zoonotic and widely spread in nature. This agent infects a wide variety of hosts, including ruminants. In bovine species, coxiellosis is responsible for reproductive disorders. Therefore, the objective of this work was to investigate the presence of Coxiella burnetii in abortions of bovine females in Brazil. In this study, 126 samples consisting of fetal pool, thymus, lymph node, central nervous system and placentas were used. The diagnosis was carried out using the quantitative PCR technique using the EXOone Coxiella burnetii® commercial kit. 20 positive samples were detected out of 126 analyzed. Of the samples that were positive, 15 were from the fetal pool, 3 from the thymus and 2 from placentas. In this study, Coxiella burnetii was detected for the first time in the fetal thymus, and in Brazil in the placenta. Given these results, it is demonstrated that the pathogen is circulating in the country and diagnostic and disease control measures must be developed. Due to the risks to public health associated with occupational activity and exposure to Coxiella burnetii, it is necessary to implement single health surveillance measures.
 
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Publishing Date
2024-04-03
 
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