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Doctoral Thesis
DOI
https://doi.org/10.11606/T.10.2022.tde-26012023-113326
Document
Author
Full name
Beatriz Martins Parra
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2022
Supervisor
Committee
Moreno, Andrea Micke (President)
Balian, Simone de Carvalho
Castro, Alessandra Marnie Martins Gomes de
Martins, Simone Maria Massami Kitamura
Menão, Marcia Cristina
Title in Portuguese
Avaliação dos agentes causadores de infecção urinária em fêmeas suínas
Keywords in Portuguese
Fêmeas suínas
Infecção urinária
Isolamento
Lesões
MALDI-TOF MS
Abstract in Portuguese
As infecções urinárias causam grandes prejuízos econômicos à suinocultura, seja com gastos medicamentosos, descarte de matrizes ou mortalidade dos animais. No Brasil, os estudos recentes envolvendo a identificação e caracterização molecular das estirpes bacterianas e fúngicas nas infecções urinárias dos suínos são escassos, bem como avaliações sobre a resistência destes microrganismos aos agentes antimicrobianos. O presente estudo teve por objetivos isolar e identificar as diferentes espécies bacterianas e fúngicas associadas à infecção urinária na espécie suína, realizar o exame anatomopatológico do rim e da bexiga dos animais avaliados, caracterizar os fatores de virulência, e determinar o padrão de resistência a antimicrobianos em estirpes de Escherichia coli isoladas. A partir dos 92 animais estudados foi identificada uma ampla variedade de espécies bacterianas em fêmeas com presença e ausência de lesão compatível com quadro de infecção urinária em bexiga e rim. Entre os principais agentes identificados estão Escherichia coli, Enterococcus spp., Staphylococcus spp., Klebsiella pneumoniae e Corynebacterium amycolatum. As estirpes de E. coli apresentaram diversos fatores de virulência associados ao patotipo ExPEC, e mais de 91% delas foram consideradas multirresistentes. Os resultados obtidos serão de grande importância no controle destas infecções nos sistemas intensivos de produção e na conscientização dos veterinários e produtores sobre o uso consciente de antimicrobianos em produção de suínos.
Title in English
Evaluation on the agents causing urinary infection in sows
Keywords in English
Injuries
Isolation
MALDI-TOF
Sows
Urinary infections
Abstract in English
Urinary infections cause great economic losses to pig farming, whether with antimicrobial costs, disposal of sows or animal mortality. In Brazil, recent studies involving the identification and molecular characterization of bacterial and fungal strains of swine urinary infections are scarce, as well as the assessments of the antimicrobials resistance of these microorganisms. The present study aims to isolate and identify the different bacterial and fungal species associated with swine urinary infection, perform the anatomopathological examination of the kidney and bladder of the evaluated animals, characterize the virulence factors in isolated Escherichia coli strains, and determine the antimicrobial resistance patterns of the most frequent bacterial species. From the 92 animals studied, a wide variety of bacterial species was identified in females with the presence and absence of lesions compatible with urinary tract infection in the bladder and kidney. Among the main agents identified are Escherichia coli, Enterococcus spp., Staphylococcus spp., Klebsiella pneumoniae and Corynebacterium amycolatum. E. coli strains showed several virulence factors associated with ExPEC pathotype, and more than 91% of them were considered multidrug-resistant. The results obtained will be of great importance in the control of these infections in intensive production systems and in the awareness of veterinarians and producers about the conscious use of antimicrobials in pig production.
 
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Publishing Date
2023-02-24
 
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