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Mémoire de Maîtrise
DOI
10.11606/D.10.2008.tde-09012009-105909
Document
Auteur
Nom complet
Daniele Cristine Raimundo
Adresse Mail
Unité de l'USP
Domain de Connaissance
Date de Soutenance
Editeur
São Paulo, 2008
Directeur
Jury
Telles, Evelise Oliveira (Président)
Knöbl, Terezinha
Moreno, Andrea Micke
Titre en portugais
Estudo comparativo entre a eficiência da filagem da Mozzarella e da pasteurização lenta do leite na inativação do Mycobacterium fortuitum e da Listeria monocytogenes experimentalmente inoculados no leite de búfala
Mots-clés en portugais
Listeria monocytogenes
Mycobacterium fortuitum
Filagem
Inativação térmica
Pasteurização
Resumé en portugais
Leite integral de búfala foi experimentalmente contaminado com Mycobacterium fortuitum e Listeria monocytogenes. Foi feita a análise quantitativa dos agentes imediatamente antes e depois de dois tratamentos térmicos: pasteurização (do leite) e filagem (da Mozzarella). Na análise da Listeria, foi empregada metodologia adaptada para a obtenção do NMP, usando os meios UVM, Fraser e Palcam como pré-enriquecimento, enriquecimento seletivo e isolamento, respectivamente. O Mycobacterium foi semeado em placas com meio Löwenstein-Jensen modificado. Os resultados mostraram que a pasteurização do leite reduziu em média 6,0 log NMP/mL de Listeria sp e 4,0 log UFC/mL de Mycobacterium sp. A filagem da coalhada reduziu em média 8,37 log NMP/g de Listeria sp e 6,3 log UFC/g de Mycobacterium sp. Concluiu-se que a filagem da Mozzarella foi mais eficiente que a pasteurização do leite na redução do inóculo de Listeria monocytogenes e Mycobacterium fortuitum em cerca de 2 logaritmos de cada agente.
Titre en anglais
Comparative study between the efficiency of the Mozzarella stretching and the holder pasteurization of milk in the inhibition of the Mycobacterium fortuitum and the Listeria monocytogenes experimentally inoculated into buffalo milk
Mots-clés en anglais
Listeria monocytogenes
Mycobacterium fortuitum
Pasteurization
Stretching
Thermal inhibition
Resumé en anglais
Whole buffalo milk was experimentally contaminated with Mycobacterium fortuitum and Listeria monocytogenes. The quantitative analysis of the agents was made immediately before and after two thermal treatments: pasteurization (of milk) and stretching (of Mozzarella). In the analysis of the Listeria, methodology adapted for the attainment of the MPN was used, employing UVM, Fraser and Palcam as not selective enrichment, selective enrichment and isolation, respectively. The Mycobacterium was plated onto modified Löwenstein-Jensen. The results had shown that the pasteurization of milk reduced 6,0 log MPN/mL Listeria sp on average, and 4,0 log CFU/mL de Mycobacterium sp. The stretching of the curd reduced 8,37 log MPN/g de Listeria sp on average, and 6,3 log CFU/g de Mycobacterium sp. It has been concluded that the stretching of Mozzarella was more efficient than the pasteurization of milk in the reduction of Listeria monocytogenes inoculum and Mycobacterium fortuitum in about 2 logarithms of each agent.
 
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Date de Publication
2009-01-27
 
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