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Master's Dissertation
DOI
10.11606/D.10.2011.tde-04092012-160643
Document
Author
Full name
Camila Peloso Bello
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2010
Supervisor
Committee
Ferreira, Antônio José Piantino (President)
Pizarro, Liliana Del Carmen Revolledo
Raso, Tânia de Freitas
Title in Portuguese
Detecção molecular e diagnóstico diferencial de vírus entéricos em aves comerciais
Keywords in Portuguese
Avicultura
Diagnóstico
Galinhas
PCR
Vírus entéricos
Abstract in Portuguese
O Brasil é omaior exportador e o terceiro maior produtor de carne de aves, atrás apenas de Estados Unidos e China. Para que o país se mantenha neste patamar e continue a crescer como produtor de frango, é fundamental que o setor avícola brasileiro priorize as questões sanitárias. Dentre as doenças que acometem as aves, um dos grupos mais importantes são aquelas que afetam o trato digestivo. As infecções intestinais em aves podem ser determinadas por bactérias, protozoários, micotoxinas e vírus. Os vírus entéricos das galinhas, incluindo Adenovírus, Astrovírus, Reovírus e Rotavírus, têm sido descritos como possíveis causadores da síndrome da má absorção (runting stunting syndrome), caracterizada principalmente por deficiência no crescimento, desenvolvimento retardado das penase diarréia. Neste estudo foram avaliadas amostras provenientes de aves de produção de diferentes origens (frangos de corte, reprodutoras e poedeiras). O conteúdo intestinal de aves oriundas de lotes com sinais clínicos entéricos e também lotes com ausência destes sinais foi submetido ao diagnóstico diferencial por PCR para quatro agentes virais: Adenovírus, Astrovírus, Reovírus e Rotavírus. Das 162 amostras testadas, 65 amostras foram positivas (40,1%) para um ou mais vírus e 97 amostras foram negativas (59,9%),sendo 2 amostras (1,2%) positivas para Adenovírus, 50 (30,9%) amostras positivas para Astrovírus, 19 (11,7%) amostras positivas para Reovírus e 12 (7,4%) foram positivas para Rotavírus.Astrovírus, Reovírus e Rotavírus parecem desempenhar papel importante como agentes causadores de problemas entéricos em aves de produção, especialmente em frangos de corte. Entretanto, mais estudos direcionados à detecção, isolamento, inoculação experimental, possíveis interações e caracterização molecular relacionados aos vírus entéricos são fundamentais para melhor esclarecimento da participação destes como agentes causadores de doença em aves.
Title in English
Molecular detection and differential diagnosis of enteric viruses from commercial chickens
Keywords in English
Chickens
Diagnostic
Enteric viruses
PCR
Poultry
Abstract in English
Brazil is the largest exporter and the world's third largest poultry produces, behind USA and China. It is essential that Brazil priorizes healthy issuesin order to maintain this position and keep growing as poultry producer. Diseases that affect the digestive system of chickens are one of the most important group of diseases in poultry flocks.There are different causes of Eenteric infections in chickens may be caused by, such as bacterias, protozoans, mycotoxins and viruses.Enteric viruses, like adenovirus, astrovirus, reovirus e rotavírus, have been described as possible cause of Runting Stunting Syndrome (RSS) in poultry, characterized by growth problems, poor feathering and diarrhea. In this study were evaluated samples from laying hens, breeders and broilers. The intestinal content from healthy and affected flocks were tested by PCR for four enteric viruses from chickens: adenovirus, astrovirus, reovirus e rotavirus. From the 162 samples tested, 65 (40,1%) were positive for one or more viruses and 97 (59,9%) were negative. Two samples (1,2%) were positive for adenovirus, 50 (30,9%) were positive for astrovirus, 19 (11,7%) were positive for reovirus and 12 (7,4%) were positive for rotavírus. Astrovirus, reovirus and rotavírusseem to play a important role as causative agent of enterical disorders in chickens, mainly in commercial broilers. However, more studies about detection, viral growth, molecular characterization, experimental inoculation and possible interaction with other agents related to enteric viruses in chickens are necessary to elucidate the participation of these viruses as causative agent of diseases in chickens.
 
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Publishing Date
2012-10-01
 
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