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Master's Dissertation
DOI
https://doi.org/10.11606/D.10.2020.tde-20092021-120910
Document
Author
Full name
Cristiano Batista Ribas
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2020
Supervisor
Committee
Ferreira, Antônio José Piantino (President)
Arango, Lynda Jhailu Tamayo
Rigoglio, Nathia Nathaly
Title in Portuguese
Estudo das alterações histopatológicas e ultraestruturais do intestino delgado de frangos infectados experimentalmente com Eimeria spp. e Clostridium perfringens para desenvolvimento de enterite necrótica
Keywords in Portuguese
Clostridium perfringens
Eimeria spp.
Enterite necrótica
Frango
Abstract in Portuguese
A enterite necrótica é uma enfermidade causada por Clostridium perfringens (C. perfringens). Infecções por Eimeria spp. pode agravar o quadro clínico desta doença, pois provocam lesões na mucosa intestinal proporcionando um ambiente mais favorável para o crescimento de C. perfringens. Demonstrar as alterações no intestino delgado provocadas por C. perfringens, Eimeria spp. ou a associação desses patógenos para a reprodução experimental da enterite necrótica em frangos foram os objetivos deste estudo. Um total de 48 animais foram utilizados, sendo divididos em 4 grupos com 12 animais em cada: Grupo Controle (T1); Grupo infectado por Eimeria spp.(T2); Grupo infectado por C. perfringens (T3) e Grupo infectado com Eimeria spp. e C. perfringens (T4). A infecção com Eimeria spp. foi realizada aos 14 dias de idade para os grupos T2 e T4 e com C. perfringens aos 19 dias de idade para os grupos T3 e T4. Foram realizadas coletas semanais de fragmentos do terço médio de duodeno, jejuno e íleo aos 22, 28, 35 e 42 dias de vida para análises histopatológicas e de microscopia eletrônica de varredura. Os grupos infectados apresentaram alterações nas vilosidades, principalmente em seus ápices. A presença de muco, danos ao epitélio de revestimento, acúmulo de detritos celulares, fusão de vilosidades e presença de edema foram os achados comuns aos grupos infectados. O grupo T4, com coinfecção de patógenos, apresentou alterações mais severas, precoces e duradouras quando comparadas a infecções com apenas Eimeria spp. ou C. perfringens.
Title in English
Study of the histopathologic and ultrastructural alterations in the small intestine of poults infected experimentally with Clostridium perfringens and Eimeria spp. for the development of necrotic enteritis
Keywords in English
Clostridium perfringens
Eimeria spp.
Broiler Chicken
Necrotic enteritis
Abstract in English
Necrotic enteritis is a disease caused by Clostridium perfringens (C. perfringens). Infections by Eimeria spp. can worsen the clinical picture of this disease, as they cause lesions in the intestinal mucosa providing a more favorable environment for the growth of C. perfringens. Demonstrate the changes in the small intestine caused by C. perfringens, Eimeria spp. or the association of these pathogens for the experimental reproduction of necrotic enteritis in chickens were the objectives of this study. A total of 48 animals were used, being divided into 4 groups with 12 animals in each: Control Group (T1); Group infected with Eimeria spp. (T2); Group infected with C. perfringens (T3) and Group infected with Eimeria spp. and C. perfringens (T4). Infection with Eimeria spp. was performed at 14 days of age for groups T2 and T4 and with C. perfringensat 19 days of age for groups T3 and T4. Weekly collections of fragments of the middle third of the duodenum, jejunum and ileum were performed at 22, 28, 35 and 42 days of life for histopathological and scanning electron microscopy analysis. The infected groups showed changes in the villi, mainly in their apexes. The presence of mucus, damage to the lining epithelium, accumulation of cellular debris, fusion of villi and the presence of edema were the common findings of the infected groups. The T4 group, with co-infection of pathogens, showed more severe, early and lasting changes when compared to infections with only Eimeria spp. or C. perfringens.
 
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Publishing Date
2021-11-26
 
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