• JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
 
  Bookmark and Share
 
 
Doctoral Thesis
DOI
10.11606/T.10.2002.tde-06082007-115734
Document
Author
Full name
Tatiana Carlesso dos Santos
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2002
Supervisor
Committee
Miglino, Maria Angélica (President)
Deschamps, João Carlos
Esper, Cesar Roberto
Souza, Wilson Machado de
Visintin, José Antonio
Title in Portuguese
A relação materno-fetal em Tayassuidae: catetos (Tayassu tajacu Linnaeus, 1758) e queixadas (Tayassu pecari Link, 1795)
Keywords in Portuguese
Tayassu
Epiteliocorial
Microvascularização
Placenta
Abstract in Portuguese
Estudaram-se a morfologia macroscópica e microscópica da placenta de 12 fêmeas adultas prenhes de cateto (Tayassu pecari) e 03 de queixada (Tayassu pecari). Os animais foram obtidos em criatórios autorizados pelo IBAMA e no Centro de Multiplicação de Animais Silvestres, Mossoró,RN. Fragmentos da placenta e do cordão umbilical foram colhidos imediatamente após a morte do animal e fixados por imersão em paraformoldeído 4% e glutaraldeído 2,5%, ambos em tampão fosfato 0,1M, para microscopia de luz, de transmissão e de reflexão, e para análise macroscópica o conjunto dos órgãos genitais foi fixado em solução aquosa de formol 10%. Para microscopia de luz, o material foi embebido em paraplast e historesina e para a microscopia eletrônica em resina Araldite®. A arquitetura microvascular foi estudada com moldes vasculares preparados a partir da combinação dos lados materno e fetal, e destes isoladamente, obtidos pela injeção de Mercox® na artéria umbilical e na artéria uterina. O cordão umbilical está composto por três vasos do tipo muscular, duas artérias e uma veia umbilical, e por um ducto alantóide. A placenta dos Taiassuídeos é corioalantóide, difusa e pregueada. O saco coriônico é fusiforme e possui superfície recoberta por pequenas pregas e aréolas. Os cortes transversais evidenciaram o epitélio uterino íntegro e o trofoblasto, caracterizando a placenta epiteliocorial. O trofoblasto é cilíndrico na base das rugas coriônicas, com células polarizadas, núcleos esféricos e citoplasma contendo numerosos vacúolos PAS+ no pólo basal, enquanto que no topo das rugas são cubóides, como o epitélio materno. Os capilares fetais possuem alta capacidade de entremearem-se no trofoblasto no topo e paredes das rugas fetais. A interface materno-fetal é PAS+, bem como o epitélio glandular e a sua secreção. As glândulas uterinas secretam na cavidade areolar material PAS+ e as células glandulares são Perls+. A barreira placentária possui todas as camadas e pode chegar a 3 micrômetros de espessura ou menos. A análise dos moldes vasculares demonstra que a relação entre sangue materno-fetal desenvolve-se de contra-corrente para corrente-cruzada. Os capilares fetais formam fileiras de protrusões bulbosas, que se encaixam nos cestos maternos formados por rugas e depressões. A placenta dos pecaris pode ainda ser classificada como adeciduada.
Title in English
The materno-fetal interrelationship in Tayassuidae: collared peccary (Tayassu tajacu Linnaeus, 1758) and white lipped-peccary (Tayassu pecari Link, 1795)
Keywords in English
Tayassu
Epitheliochorial
Microvascularization
Placenta
Abstract in English
Macro and microscopic morphology in placenta of the twelve collared peccary (Tayassu pecari) and three white-lipped peccary (Tayassu pecari) were studied. The animals were obtained from authorized farms by IBAMA and from CEMAS, Mossoró, RN. Fragments from placenta and from umbilical cord were obtained immediately after slaughter and fixed by immersion in 4% paraformoldehyde and 2.5% glutaraldehyde in 0.1M phosphate buffer, to light, transmission and scanning electron microscopy analysis and the genital organs were fixed in aqueous solution of formaldehyde 10% to macroscopic analysis. For light microscopy, the pieces of organs were embedded in paraplast or historesin and to transmission electron microscopic were embedded in Araldit® resin. The microvascular architecture was studied with blood vessels casts prepared from de maternal, fetal and combined maternal and fetal sides, by Mercox® injection into the umbilical artery and uterine artery. The umbilical cord has three muscular vessels, two arteries and one umbilical vein, and one alantoic duct. The placenta of Tayassuidae is chorioalantoid, diffuse and folded. The chorionic sac is fusiform and its surface is covered with folds and small areolas. The transversal sections show the presence of the trophoblast and maternal epithelium, characterizing the epitheliochorial placenta. The trophoblast is columnar on the base of fetal rugae and contains polarized cells, spherical nucleus and cytoplasm with many vacuoles with material PAS + in the basal polo, while on the top of rugae the trophoblast is cuboid as the uterine epithelium. The capillaries have characteristics of the indentation between trophoblast cells on the top and side of the fetal rugae. The materno-fetal interface is PAS+, as well as the glandular epithelium and their secretion. The uterine glands secreted in the areolar cavity PAS+ material and their cells are Perls+. The interhemal barrier has all layers and may have three micrometers or less. The analysis of the vessels casts shows that materno-fetal blood interrelationship develops into a crosscurrent to countercurrent. The fetals capillaries form rows of bulbous protrusions fitting into the complementary maternal baskets formed by ridges and troughs. The placenta of peccaries still may be classified as adeciduade.
 
WARNING - Viewing this document is conditioned on your acceptance of the following terms of use:
This document is only for private use for research and teaching activities. Reproduction for commercial use is forbidden. This rights cover the whole data about this document as well as its contents. Any uses or copies of this document in whole or in part must include the author's name.
Publishing Date
2007-08-08
 
WARNING: Learn what derived works are clicking here.
All rights of the thesis/dissertation are from the authors
Centro de Informática de São Carlos
Digital Library of Theses and Dissertations of USP. Copyright © 2001-2022. All rights reserved.