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Master's Dissertation
DOI
10.11606/D.10.2010.tde-06072012-104345
Document
Author
Full name
Isabella Rodrigues Fernandes
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2010
Supervisor
Committee
Braga, Patricia Cristina Baleeiro Beltrão (President)
Miglino, Maria Angélica
Pignatari, Graciela Conceição
Title in Portuguese
Cultura e caracterização de células do trofoblasto extraviloso (TEV) derivado da placenta humana a termo
Keywords in Portuguese
Célula placentária
Célula-tronco
Placenta a termo
Terapia celular
Trofoblasto extraviloso
Abstract in Portuguese
A placenta é um anexo embrionário que tem atraído grande interesse como fonte de células-tronco para medicina regenerativa, devido à plasticidade fenotípica de alguns dentre os vários tipos celulares isolados a partir deste tecido. Apesar de terem a mesma origem, não fazem parte do embrião, portanto o uso da placenta como fonte de células embrionárias não provoca debates éticos. Uma característica que vale a pena mencionar, é que a placenta está envolvida na manutenção da tolerância do feto pelo organismo materno, pois contém células que apresentam propriedades imunomoduladoras. Por fim, o tecido placentário é disponibilizado após o parto e é geralmente descartado. Estas características tornam esse tecido de grande interesse para protocolos de terapia celular, tanto que tem surgido bancos de célulatronco de placenta humana. Alguns trabalhos demonstraram plasticidade de células extraídas da placenta, porém existe ainda a necessidade de se definir melhor a região de coleta e os métodos de extração e isolamento dessas células. Nosso grupo estabeleceu a cultura de células derivadas da região do trofoblasto extraviloso (TEV) de placenta humana a termo, que são as células responsáveis pelos mecanismos de imunotolerância materno-fetal. As células TEV apresentam os marcadores de pluripotencia Oct-4 e Nanog, e, portanto, podem reter mesmo extraídas da placenta a termo, alguma plasticidade celular, caracterizando-as como células-tronco. Entretanto, nossa experiência no cultivo destas células mostrou que existem limitações relacionadas ao tempo de cultivo celular e capacidade de proliferação das TEV, que certamente fornece argumentos sólidos para limitar o seu uso em protocolos de terapia celular em medicina regenerativa.
Title in English
Culture and characterization of extravillous trophoblast cells (EVT) derived from human term placenta
Keywords in English
Cell therapy
Extravillous trophoblast
Placenta at term
Placental Cell
Stem cell
Abstract in English
The placenta is attached embryo that has attracted great interest as a source of stem cells for regenerative medicine due to phenotypic plasticity of some of the various cell types isolated from this tissue. Despite having the same origin, they are not part of the embryo, so the use of placenta as a source of embryonic cells does not provoke ethical debates. A feature worth mentioning is that the placenta is involved in maintaining tolerance of the fetus by the mother, because it contains cells that have immunomodulatory properties. Finally, the placental tissue is present after birth and is usually discarded. These characteristics make this fabric of great interest for cell therapy protocols, which has emerged both banks of stem cells from human placenta. Some studies have demonstrated plasticity of cells extracted from the placenta, but there is still a need to better define the catchment area and the methods of extraction and isolation of these cells. Our group has established a culture of cells derived from the extravillous trophoblast region (TEV) from human placenta at term, which are the cells responsible for the mechanisms of maternalfetal immunotolerance. TEV cells have the pluripotency markers Oct-4 and Nanog, and therefore can retain, even extracted from the placenta at term, some cellular plasticity, characterizing them as stem cells. However, our experience in growing these cells showed that there are limitations related to the time of cell culture and proliferation capacity, which certainly provides strong arguments for limiting their use in cell therapy protocols in regenerative medicine.
 
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Release Date
2017-04-25
Publishing Date
2013-04-26
 
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