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Master's Dissertation
DOI
10.11606/D.10.2006.tde-04042007-151336
Document
Author
Full name
Vivian Yochiko Samoto
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2006
Supervisor
Committee
Miglino, Maria Angélica (President)
Carvalho, Ana Flávia de
Stopiglia, Angelo João
Title in Portuguese
Terapia celular cardíaca: vias de infusão de células mononucleares em cães SRD
Keywords in Portuguese
Cães
Células mononucleares
Coração
Medula óssea
Vias de infusão
Abstract in Portuguese
As células-tronco adultas possuem capacidade de se transformar em certos tipos de tecidos. Surge desta forma, uma nova modalidade de tratamento para doenças cardíacas, assim, uma avaliação na distribuição e quantificação do número de células por diferentes vias de infusão tornam-se importantes, pois o microambiente e interação célula/célula interferem no processo de transdiferenciação e/ou fusão. Foram utilizados nove cães, com peso entre 25 e 30kg e hígidos, no qual foi realizada punção, separação, marcação e infusão de células mononucleares da medula óssea pelas vias intracoronariana, transendocárdica e venosa retrógrada. Pôde-se observar a presença de células por todo tecido cardíaco em todas as vias, contudo o padrão de distribuição das mesmas difere, no qual há um predomínio de células em tecido conjuntivo, principalmente em epicárdio nas vias transendocárdica e venosa retrógrada e um padrão intersticial muscular intracoronariana. Há diferenças significativas na quantidade de células nas regiões apical, basal, medial e apical quando relacionada à via de infusão (P<0,01).
Title in English
Cardiac cellular therapy: pathway of mononuclear stem cell infusion in mongrel dogs
Keywords in English
Bone marrow
Heart
Mongrel dogs
Mononuclear cells
Routes of infusion
Abstract in English
The adult stem cells have a capacity to transform in different kinds of tissue. It is emerges like a new treatment modality of cardiac diseases, so, an evaluation in cell distribution and quantification by different routes of infusion in heart are necessary, because the microenvironment and the interaction cell/cell age in the transdifferentiation and fusion process. It was used 09 healthy mongrel dogs, with 25 -30kg of weight, which was performed a punction of iliacal crest, bone marrow mononuclear cells separation by Ficoll density, stained with Hoechst and infusion by intracoronary, transendocardial and retrograde routes. The marked cells was observed in all cardiac tissue, however, the pattern of distribution of the same ones differs, however in the transendocardial and retrograde route the cells could be founded mainly into the connective tissue - epicardium and sub-epicardic connective tissue and in the intracoronary route, the cells was observed mainly in the muscular interstice. It was observed a significant number of cells in the atrial, basal, medium and apical cardiac region when related with the route of infusion.
 
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VivianSamoto.pdf (6.02 Mbytes)
Publishing Date
2007-04-09
 
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