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Master's Dissertation
DOI
https://doi.org/10.11606/D.9.2017.tde-09082017-145026
Document
Author
Full name
Fabio Kummrow
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2001
Supervisor
Committee
Umbuzeiro, Gisela de Aragão (President)
Di Mascio, Paolo
Fernícola, Nilda Alícia Galego Gandara de
Title in Portuguese
Blue rayon como alternativa para extração de compostos orgânicos genotóxicos presentes em amostras de águas
Keywords in Portuguese
Água (Análise química)
Água Contaminada (Análise química)
Blue rayon
Compostos orgânicos (Toxicidade)
Genotóxicos
Toxicologia Ambiental
Abstract in Portuguese
A combinação do teste de Ames e métodos seletivos de extração para os principais grupos de compostos responsáveis pela atividade genotóxica em amostras ambientais tem sido muito utilizada. O objetivo foi validar o uso do Blue rayon como alternativa para extração de compostos presentes em águas brutas e tratadas. Foram realizados experimentos comparativos entre o Blue rayon e XAD-4 com amostras fortificadas e amostras reais. Dois mananciais, um contaminado com compostos policíclicos e outro não, foram estudados. As amostras foram extraídas com resina XAD4, e com Blue rayon, este último seletivo para compostos policíclicos. Os extratos foram testados com as linhagens de Salmonella typhimurium TA98 e TA100 na presença e ausência de mistura S9. O Blue rayon foi menos eficiente na detecção da mutagenicidade total das amostras quando comparado com a XAD-4. Porém foi capaz de distinguir a mutagenicidade já presente na água bruta daquela gerada durante o tratamento.
Title in English
Blue Rayon as an alternative for extraction of genotoxic organic compounds present in water samples
Keywords in English
Blue rayon
Contaminated water (Chemical analysis)
Environmental toxicology
Genotoxics
Organic compounds (Toxicity)
Water (Chemical analysis)
Abstract in English
The combination of the Ames test and selective extraction methodologies has been successfully used to indicate the possible classes of organic contaminants in environmental samples. The aim of this study was to validate the use of Blue rayon as an alternative extraction method to detect compounds in raw and treated waters. Two different water bodies were evaluated and in one of the sites, raw waters were known to be contaminated with polycyclic compounds. Experiments comparing the Blue rayon technique with XAD-4 resin were done in environmental and spiked samples. The extracts were tested for mutagenicity with Salmonella typhimurium strains TA98 and TA100, with and without metabolic activation. Blue rayon was less efficient in detecting the overall mutagenicity of the samples analyzed in comparison with XAD-4. It was able though to distinguish the mutagenicity due to the contaminants in raw from the ones generated by water treatment.
 
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Publishing Date
2017-08-09
 
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