Avaliação de regiões hipervariáveis de genes que predispõem à obesidade

Hinuy, Hamilton Massayuki

doi:10.11606/D.9.2004.tde-31082006-215706

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Master's Dissertation

DOI

https://doi.org/10.11606/D.9.2004.tde-31082006-215706

Document

Master's Dissertation

Author

Hinuy, Hamilton Massayuki (Catálogo USP)

Full name

Hamilton Massayuki Hinuy

Institute/School/College

Faculdade de Ciências Farmacêuticas

Knowledge Area

Clinical Analysis

Date of Defense

2004-04-02

Published

São Paulo, 2004

Supervisor

Hirata, Rosario Dominguez Crespo (Catálogo USP)

Committee

Hirata, Rosario Dominguez Crespo (President)
Gomes, Ligia Ferreira
Villares, Sandra Mara Ferreira

Title in Portuguese

"Avaliação de regiões hipervariáveis de genes que predispõem à obesidade"

Keywords in Portuguese

IMC
leptina
microssatélites
obesidade
polimorfismo genético

Abstract in Portuguese

As variantes genéticas LEP G-2548A, LEP 3'HVR, D1S200 (LEPR),D18S858 (MC4R) e D2S1788 (POMC)foram avaliadas em 100 indivíduos obesos (GE) e 110 não-obesos (GC) brancos. A genotipagem desses indivíduos foi realizada por PCR e RFLP. As freqüências dos alelos LEP -2548G e LEP 3'HVR-Classe I no grupo GE foram maiores que no GC P<0,05). O haplótipo LEP G/I foi mais freqüente no grupo GE (P=0,018) e nos indivíduos com obesidade central (P=0,047). As freqüências dos alelos 41/42 da D18S858 e do alelo 17 da D1S200 foram maiores (P<0,05) no grupo GE. Os indivíduos com alelos LEP 3'HVR-Classe I, 41/42 da D18S858 e 17 da D1S200 apresentaram valores mais altos de índice de massa corporal (IMC) e circunferência abdominal (P<0,05). Em conclusão, as variantes LEP G-2548A, LEP 3'HVR, D18S858 e D1S200 estão associadas com a obesidade e com variações nos valores de IMC e circunferência abdominal em indivíduos brasileiros brancos.

Title in English

Evaluation of hypervariable regions from genes predisposing to obesity

Keywords in English

BMI
genetic polymorphism
leptin
microsatellite
obesity

Abstract in English

The genetic variants LEP G-2548A, LEP 3'HVR, D1S200 (LEPR), D18S858 (MC4R) D2S1788 (POMC) were studied in groups of 100 obese (GE) and 110 non-obese (GC) white individuals. Genotyping were carried out by PCR and RFLP techniques. Alleles frequencies from LEP -2548G and Class I alleles from LEP 3'HVR were higher in GE group, when compared to GC group (P<0,05). The LEP G/I haplotype was more frequent in GE group (P=0,018) and in individuals with central obesity (P=0,047). Alleles 41/42 from D18S858 and allele 17 from D1S200 were more frequent (P<0,05) in GE group. Individuals carrying LEP 3'HVR-Class I, alleles 41/42 from D18S858 and allele 17 from have shown elevated body mass index (BMI) and waist circumference values (P<0,05). In conclusion, the LEP G-2548A, LEP 3' HVR, D1S200, and D18S858 genetic variants were found to be associated to obesity and variations in BMI and waist circumference in Brazilian white individuals.

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Publishing Date

2006-11-23

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