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Master's Dissertation
DOI
https://doi.org/10.11606/D.9.2017.tde-19122017-170534
Document
Author
Full name
Andreia Ricci Hernandes
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2005
Supervisor
Committee
Cordenunsi, Beatriz Rosana (President)
Mercier, Helenice
Purgatto, Eduardo
Title in Portuguese
Perfil metabólico da banana durante o amadurecimento
Keywords in Portuguese
Banana
Bioquímica de alimentos
Ciência de alimentos
Espectrometria de massas
Metabólitos
Abstract in Portuguese
A análise do perfil metabólico de frutos em amadurecimento pode prover informações a respeito de quais as vias metabólicas são ativadas e inativadas durante o processo. Tais informações podem auxiliar na identificação de genes expressos nessa fase do ciclo de vida do fruto, ampliando o conhecimento sobre os mecanismos envolvidos no amadurecimento. Usando esta abordagem, este trabalho tem por objetivo identificar mudanças significativas nos níveis de metabólitos da banana durante o amadurecimento induzido pelo etileno. Para tal utilizou-se a cromatografia em fase gasosa acoplada a espectrometria de massas para a análise em função do poder de resolução da técnica. Utilizando a biblioteca de espectros de massa NIST 98 foi possível identificar 70 substâncias na fração polar e 40 substâncias na fração apoiar incluindo aminoácidos, açúcares, ácidos orgânicos, lipídeos entre outros. A análise de perfil metabólico revelou importantes variações nos níveis metabólicos de açúcares e ácidos orgânicos, provavelmente devido ao aumento na taxa respiratória do fruto. A maltose e o mio-inositol foram os metabólitos escolhidos para uma análise mais detalhada, que evidenciou a influência de outros metabólitos independentes do etileno na regulação dos eventos da degradação do amido.
Title in English
Metabolic profile of banana during ripening
Keywords in English
Banana
Food biochemistry
Food science
Mass spectrometry
Metabolites
Abstract in English
The analysis of the metabolic profile of fruits can provide information regarding which metabolic ways are activated and inactivated during the ripening. Such information may help the identification of expressed genes in this process extending the knowledge on the involved mechanisms of ripening. Using this approach, the aim of this work was identify significant changes in the levels of metabolites of the banana during the ripening induced by ethylene. In this way it was used gas chromatography connected the mass spectrometry for these analysis, due to the high resolution of this technique. Using the NIST 98 library of mass spectra it was possible to identify about 70 substances in the polar fraction and 40 substances in the apolar fraction, including amino acids, sugars, organic acid, lipids, and others. The analysis of metabolic profile revealed important variations in the metabolic levels of organic acid sugars and, probably due to an increase in the respiratory rate of the fruit. A detailed analysis was conducted with maltose and myo-inosytol and revealed the influence of other metabolites which independed of ethylene, in the regulation of starch degradation.
 
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Publishing Date
2017-12-19
 
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