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Master's Dissertation
DOI
https://doi.org/10.11606/D.9.2007.tde-05122007-151058
Document
Author
Full name
Claudia Mitsue Koike
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2007
Supervisor
Committee
Purgatto, Eduardo (President)
Carvalho, Maria Angela Machado de
Cordenunsi, Beatriz Rosana
Title in Portuguese
Variação hormonal correlacionada à expressão de enzimas ligadas ao metabolismo do amido durante o desenvolvimento e amadurecimento da manga (Mangifera indica cv Keitt)
Keywords in Portuguese
Amido
Beta-amilase
Fitormônios
Manga
Mangifera Indica
Abstract in Portuguese
O desenvolvimento e o amadurecimento de frutos são processsos complexos e organizados, ondeo fitormônios, como o ácido indol-3-acético (AIA), o ácido abscísico (ABA) e o etileno desempenham papel relevante. Dentro os processos característicos do amadurecimento, a degradação do amido apresenta impacto direto sobre a qualidade da manga, sendo practicamente todo degradao a síntese de sacarose. Várias enzimas participam da degradação do grânulo de amido, como também em bananas. A mobilização do amido em mangas Keitt parece estar dissociada temporalmente da evolução do etileno; e as variações nos níveis de AIA e ABA sugerem um possível papel na sinalização para mobilização do amido. A biossíntese da sacarose foi atrasada pelo 1-MCP, um antagonista do etileno, sugerindo redução na velocidade do fluxo de carbono do amido para síntese da sacarose que pode ser, resultante das alterações no metabolismo da maltose proveniente da degradação do amido.
Title in English
Correlation with hormonal variation and genes expression of enzimes involved in starch metabolic during during development and ripening of mango (Mangifera indica cv Keitt)
Keywords in English
Beta-amylase
Mangifera indica
Mango
Phytohormones
Starch
Abstract in English
The fruit development and ripening are organized and complex processes where phytohormones, like indol-3-acetic acid (IAA), abscisc acid (ABA) and ethylene have a pivotal role. In mango fruit one of the main metabolisms that have direct impact in the mango quality is the starch degradation, practically being all degrated the synthesis of sacarose. Some enzymes participate of the degradation, as hidrolases (alfa and beta-amylases, disproportionating enzymes) and phosphorylases, described also in bananas. The mobilization of the starch in Keitt mangoes it does not seem to be temporally associated with ethylene; and variations in the IAA and ABA levels suggest their possible role in the signalling for the starch. Sucrose biosynthesis was delayed by 1-MCP indicating a reduction in the speed of the carbon flux from the starch that it can be, resultant of the alterations in the metabolism of maltose provenient from the starch degradation.
 
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Dissertacao.pdf (1.26 Mbytes)
Publishing Date
2009-01-29
 
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