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Doctoral Thesis
DOI
https://doi.org/10.11606/T.87.2010.tde-30092010-084910
Document
Author
Full name
Regina Maria Mourão Fuches
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2010
Supervisor
Committee
Sant'Anna, Osvaldo Augusto Brazil Esteves (President)
Astray, Renato Mancini
Gallina, Neuza Maria Frazatti
Polakiewicz, Bronislaw
Stephano, Marco Antonio
Title in Portuguese
Validação de bioensaios para o estudo da imunogenicidade da vacina contra raiva.
Keywords in Portuguese
Bioensaios
Esquemas de imunização
Imunogenética
Silíca nanoestruturada SBA-15
Silício
Vacina anti-rábica
Validação
Abstract in Portuguese
Para atestar a imunogenicidade de vacinas contra raiva, os testes de titulação e de soroneutralização de vírus rábico em células BHK-21 foram validados quanto à linearidade, precisão, exatidão e robustez. Cinco esquemas de imunização com diferentes concentrações, vias de inoculação e intervalos entre as doses foram avaliados em camundongos com vacina contra raiva em células Vero. O grupo II que recebeu um esquema de 2 doses e intervalos maiores (0 e 60 dias) apresentou títulos de anticorpos neutralizantes [ACN] mais elevados (46,1 UI/mL) do que o Grupo I (19,4 UI/mL), de 3 doses e intervalos menores (0,7 e 28 dias). O grupo III, que recebeu 2 doses, sendo a 1ª diluída 1/10, apresentou ACN semelhantes ao grupo II (39,2 UI/mL), sendo igualmente eficaz. Nenhum animal do grupo IV, imunizado por via oral (VO) apresentou ACN, indicando supressão e todos os do grupo V, imunizados VO com antígeno adsorvido/encapsulado em sílica nanoestruturada SBA-15, apresentaram títulos de ACN detectáveis, mostrando que o antígeno foi protegido da degradação no trato gastrointestinal.
Title in English
Validation of bioassays to immunogenicity assessment of the anti-rabies vaccines.
Keywords in English
Bioassays
Immunizine schemes
Immunogenetics
Nanostructured silica SBA-15
Rabies vaccine
Silicon
Validation
Abstract in English
To assure the reliability of the results of immunogenicity of rabies vaccines, tests of virus titration and neutralization in BHK-21 cells were validated for linearity, precision, accuracy and robustness. Five schemes of immunization with different concentrations, routes of inoculation and intervals between doses were evaluated in mice with rabies vaccine in Vero cells. Group II, immunized with 2 doses and enlarged interval (0 and 60 days) presented higher levels of neutralizing antibody (NAs) (46,1 IU/mL) than group I (19,4 IU/mL), with 3 doses and shorter intervals (0,7 and 28 days). Group III, which received 2 doses, the 1st diluted to 1/10, presented results similar to group II (39,2 IU/mL). None mouse of Group IV, immunized by oral route, presented NAs, indicating suppression and all mice of group V, immunized by oral route with vaccine adsorbed/encapsulated in nanostructured SBA-15 silica presented detectable NAs titers, showing that the SBA-15 silica prevented the antigen degradation in the gastrointestinal tract.
 
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Publishing Date
2010-11-16
 
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